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Factors associated with the collection of isolated immature oocytes during ovarian tissue cryopreservation

  • Fertility Preservation
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To identify patient characteristics associated with successful isolated immature oocyte retrieval (IsO) during ovarian tissue cryopreservation (OTC) and to determine whether they are predictive of the collection of larger numbers of oocytes.

Methods

We retrospectively analyzed all patients undergoing OTC with IsO for fertility preservation over three years of activity at a university hospital. Univariate and multivariate analyses were used to identify the patients with the highest and lowest chances of oocyte recovery, and those with the largest numbers of oocytes. We also analyzed the correlation of IsO with the number of ovarian fragments collected and histological parameters.

Results

We analyzed 257 consecutive patients undergoing these procedures, at a median age of 17.1 years [0.3–38.3 years]. Isolated oocytes were obtained from 47.1% of patients, and IsO was more likely in patients with ovulatory cycles (63.0% vs. 38.6%; P≤ .001), without chemotherapy before OTC (61.4% vs. 33.1; P< .001) and with non-malignant diseases other than Turner syndrome (77.5%). Oocyte collection failure rates were highest in patients with Turner syndrome (OR 25.0, 95% CI 3.99–157.0; P< .001) or undergoing chemotherapy with alkylating agents before OTC (OR 37.6, 95% CI 8.36–168.8; P< .001). Prepubescent status (P= .043) and large numbers of ovarian fragments (P< .001) were associated with the retrieval of larger numbers of oocytes. Oocyte recovery was correlated with the presence of follicles in the medulla, but not with follicular density.

Conclusion

The chances of IsO differ between patients. Identifying patients with the highest chances of success facilitates appropriate resource allocation.

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Acknowledgements

The authors thank Corinne Journo and Lilia Grira for technical assistance.

Author information

Author notes

  1. Marie Prades

    Present address: Department of Reproductive Biology, Tenon Hospital AP-HP, 4 rue de la Chine, 75020, Paris, France

  2. Flora Marzouk

    Present address: Laboratoire d’Analyses Médicales Drouot, 21 rue Drouot, 75009, Paris, France

  3. Benoit Schubert

    Present address: Eurofins-Biomnis Laboratory, Site du Val d’Ouest—Institut Rhônalpin-39, chemin de la Vernique, 69130, Ecully, France

  4. Catherine Genestie

    Present address: Department of Pathology, Gustave Roussy Institute, 94800, Villejuif, France

  5. Leslie Sitbon

    Present address: Department of Reproductive Biology, Bioclinic-Biomega, Centre Hospitalier Intercommunal, 40 avenue de Verdun, 94000, Créteil, France

  6. Catherine Poirot

    Present address: Department of Hematology, AYA Unit, Saint Louis Hospital AP-HP, 1 avenue Claude Vellefaux, 75010, Paris, France

  7. Nicolas Boissel, Catherine Poirot, Marie Prades and Flora Marzouk contributed equally to this work.

Authors and Affiliations

  1. Department of Reproductive Biology, University Hospital of Pitié-salpêtrière-Charles Foix, 83 boulevard de l’Hopital, 75013, Paris, France

    Marie Prades

  2. Department of Reproductive Biology, University Hospital of Pitié-salpêtrière-Charles Foix, 83 boulevard de l’Hopital, 75013, Paris, France

    Flora Marzouk

  3. Department of Reproductive Biology, University Hospital of Pitié-salpêtrière-Charles Foix, 83 boulevard de l’Hopital, 75013, Paris, France

    Benoit Schubert

  4. Department of Pathology, University Hospital of Pitié-salpêtrière-Charles Foix, 83 boulevard de l’Hopital, 75013, Paris, France

    Catherine Genestie

  5. Department of Reproductive Biology, University Hospital of Pitié-salpêtrière-Charles Foix, 83 boulevard de l’Hopital, 75013, Paris, France

    Leslie Sitbon

  6. Department of Obstetric Gynecology, University Hospital of Pitié-Salpêtrière-Charles Foix, 83 Boulevard de l’Hôpital, 75013, Paris, France

    Anne Fortin

  7. Department of Hematology, AYA Unit, Saint Louis Hospital AP-HP, 1 avenue Claude Vellefaux, 75010, Paris, France

    Nicolas Boissel

  8. Université Paris Cité, UFR Médecine, Site Villemin, 10 avenue de Verdun, EA3518, 75010, Paris, France

    Nicolas Boissel

  9. Department of Reproductive Biology, University Hospital of Pitié-salpêtrière-Charles Foix, 83 boulevard de l’Hopital, 75013, Paris, France

    Catherine Poirot

  10. Médecine Sorbonne Université, Site Pitié Salpêtrière, 91 Boulevard de l’Hôpital, 75013, Paris, France

    Catherine Poirot

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  1. Marie Prades
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  2. Flora Marzouk
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Contributions

MP, FM, NB, and CP designed the study and contributed to the literature search, data collection, data interpretation, writing, and revision of the manuscript. NB performed statistical analysis and created the figure used in the manuscript. MP, FM, BS, LS, CJ, LG undertook the preparation, freezing and cryopreservation of the ovarian tissue, and the retrieval of isolated oocytes. They contributed to data collection and interpretation. AF was responsible for the ovarian tissue pick-up program. CG performed all the histological studies.

All the authors contributed to the report through review and discussion, and all agree to publication of the manuscript.

Corresponding author

Correspondence to Marie Prades.

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The authors declare no competing interests.

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Prades, M., Marzouk, F., Schubert, B. et al. Factors associated with the collection of isolated immature oocytes during ovarian tissue cryopreservation. J Assist Reprod Genet 40, 2799–2807 (2023). https://doi.org/10.1007/s10815-023-02948-z

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  • DOI: https://doi.org/10.1007/s10815-023-02948-z

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