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Loss of PMFBP1 Disturbs Mouse Spermatogenesis by Downregulating HDAC3 Expression

  • Gamete Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Polyamine modulating factor 1 binding protein (PMFBP1) acts as a scaffold protein for the maintenance of sperm structure. The aim of this study was further to identify the new role and molecular mechanism of PMFBP1 during mouse spermatogenesis.

Methods and Results

We identified a profile of proteins interacting with PMFBP1 by immunoprecipitation combined with mass spectrometry and demonstrated that class I histone deacetylases, particularly HDAC3 and chaperonin-containing TCP1 subunit 3 (CCT3), were potential interaction partners of PMFBP1 based on network analysis of protein-protein interactions and co-immunoprecipitation. Immunoblotting and immunochemistry assays showed that loss of Pmfbp1 would result in a decline in HDACs and change the proteomic profile of mouse testis, in which differently expressed proteins are associated with spermatogenesis and assembly of flagella, which was proved by proteomic analysis of testis tissue obtained from Pmfbp1−/− mice. After integrating with transcriptome data for Hdac3−/− and Sox30−/− round sperm obtained from a public database, RT-qPCR confirmed ring finger protein 151 (Rnf151) and ring finger protein 133 (Rnf133) were key downstream response factors of the Pmfbp1-Hdac axis affecting mouse spermatogenesis.

Conclusion

Taken together, this study indicates a previously unidentified molecular mechanism of PMFBP1 in spermatogenesis whereby PMFBP1 interacts with CCT3, affecting the expression of HDAC3, followed by the downregulation of RNF151 and RNF133, resulting in an abnormal phenotype of sperm beyond the headless sperm tails. These findings not only advance our understanding of the function of Pmfbp1 in mouse spermatogenesis but also provide a typical case for multi-omics analysis used in the functional annotation of specific genes.

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Data Availability

All data are available in the main text or the supporting information materials.

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Acknowledgements

We thank Yin H. et al., Bai S. et al., and Zhang D. et al. for the transcriptomic data and ChIP-seq data (numbers GSE153065 and GSE113073) and the National Omics Data Encyclopedia (accession number OEP000012). The ChIP-seq data in this study were generated from Sox30−/− RS using the Hdac3 antibody and wild-type mouse RS using the Sox30 antibody and downloaded from the Gene Expression Omnibus (GSE153065) database. We thank Liwen Bianji (Edanz) (www.liwenbianji.cn) for editing the English text of a draft of this manuscript.

Funding

This work was supported by the National Natural Science Foundation of China (81972641 to F.W.; 82071701 to F.Z.), the Scientific Research Foundation of the Institute for Translational Medicine of Anhui Province (2021zhyx-C25), the Basic and Clinical Cooperative Research Promotion Program of Anhui Medical University (2022xkjT015), and the Natural Science Research Project for Anhui Universities (KJ2021A0242).

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Author notes

  1. Weilong Xu, Zhoujuan Yao, Yunzhi Li, and Ke Wang contributed equally to this work.

Authors and Affiliations

  1. School of Life Science, Anhui Medical University, Hefei, 230022, China

    Weilong Xu, Zhoujuan Yao, Yunzhi Li, Ke Wang, Shuai Kong, Fengsong Wang & Hui Zhang

  2. Reproductive Medicine Center, Anhui No. 2 Provincial People’s Hospital, Hefei, 230041, Anhui, China

    Ke Wang

  3. Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, 230022, Anhui, China

    Yu Wang, Mingfei Xiang & Fuxi Zhu

  4. Reproductive Medicine Center, Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Anhui Medical University, Hefei, 230601, Anhui, China

    Fuxi Zhu

  5. NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract (Anhui Medical University), Hefei, 230032, Anhui, China

    Fuxi Zhu

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  1. Weilong Xu
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Contributions

F. Wang, F. Zhu, and H. Zhang designed the research. W. Xu, Y. Li, Z. Yao, and K. Wang performed the research. S. Kong, Y. Wang, and M. Xiang analyzed data. H. Zhang and W. Xu wrote the paper. F. Wang and F. Zhu revised the manuscript.

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Correspondence to Fuxi Zhu, Fengsong Wang or Hui Zhang.

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Xu, W., Yao, Z., Li, Y. et al. Loss of PMFBP1 Disturbs Mouse Spermatogenesis by Downregulating HDAC3 Expression. J Assist Reprod Genet 40, 1865–1879 (2023). https://doi.org/10.1007/s10815-023-02874-0

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