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The effect of MicroRNAs variants on idiopathic recurrent pregnancy loss

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Abstract

Background

Although the importance of miRNA variants in female reproductive disorders has been frequently reported, the association between miRNA polymorphisms and recurrent pregnancy loss (RPL) has been poorly studied. In this study, we aimed to assess the correlation of four different miRNA variants to unexplained RPL.

Methods and results

The prevalence of four SNPs including miR-21 rs1292037, miR-155-5p rs767649, miR-218–2 rs11134527, and miR-605 rs2043556 in 280 cases with iRPL and 280 controls was performed. The DNA was extracted from all subjects and the SNPs were genotyped using RFLP-PCR methods. The data revealed that rs1292037 and rs767649 were significantly associated with higher rates of iRPL in patients compared with controls while rs11134527 and rs2043556 showed no association with increased rates of iRPL among patients. The haplotypes T-A-G-G and T-A-G-A were the most frequent in both cases and controls. Three haplotypes including T-T-G-A, C-T-G-G, and T-A-A-A showed significantly different frequencies in patients in comparison to healthy females.

Conclusion

This study suggests that rs1292037 and rs767649 could be risk factors for increased rates of iRPL.

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Data availability

The original contributions presented in the study are included in the article, further inquiries can be directed to the corresponding author.

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Authors and Affiliations

  1. Department of Obstetrics, Hengshui People’s Hospital, 180 East Renmin Rd, Hengshui, 053000, China

    Chunlei Guo, Xuejing Yin & Shuiping Yao

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  1. Chunlei Guo
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  2. Xuejing Yin
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  3. Shuiping Yao
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Correspondence to Shuiping Yao.

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This study was performed in line with the principles of the Declaration of Helsinki. This study was confirmed by the ethics review board of the Department of Obstetrics, Hengshui People’s Hospital with a full ethical code (2020–0041-25).

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Guo, C., Yin, X. & Yao, S. The effect of MicroRNAs variants on idiopathic recurrent pregnancy loss. J Assist Reprod Genet 40, 1589–1595 (2023). https://doi.org/10.1007/s10815-023-02827-7

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