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Effects of oocyte vitrification on gene expression in the liver and kidney tissues of adult offspring

  • Reproductive physiology and disease
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Oocyte vitrification is an important assisted reproductive technology (ART) that preserves the fertility of unmarried patients with malignant tumors, and promotes the development of the oocyte donation program. In recent years, the effects of ART, including the vitrification of oocytes and embryos on the health of offspring, have attracted much attention; however, it is difficult to conduct long-term follow-up and biochemical evaluation in humans. In this study, we detected the effect of oocyte vitrification on gene expression in the organs of adult mice offspring by RNA sequencing for the first time. Our results showed that only a small amount of gene expression was significantly affected. Seven genes (Tpm3, Hspe1-rs1, Ntrk2, Cyp4a31, Asic5, Cyp4a14, Retsat) were abnormally expressed in the liver, and ten genes (Lbp, Hspe1-rs1, Prxl2b, Pfn3, Gm9008, Bglap3, Col8a1, Hmgcr, Ero1lb, Ifi44l) were abnormal in the kidney. Several genes were related to metabolism and disease occurrence in the liver or kidney. Besides, we paid special attention to the expression of known imprinted genes and DNA methylation–related genes in adult organs, which are susceptible to oocyte cryopreservation in the preimplantation stage. As a result, some of these transcripts were detected in adult organs, but they were not affected by oocyte vitrification. In conclusion, we first report that oocyte vitrification did not significantly change the global gene expression in offspring organs; nonetheless, it can still influence the transcription of a few functional genes. The potential adverse effects caused by oocyte vitrification need attention and further study.

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Abbreviations

ART:

Assisted reproductive technology

ICSI:

Intracytoplasmic sperm injection

RNA-seq:

RNA sequencing

TG:

Triglyceride

COCs:

Cumulus-oocyte complexes

ES:

Equilibrium solution

EG:

Ethylene glycol

DMSO:

Dimethyl sulfide

VS:

Vitrification solution

TS:

Thawing solution

DS:

Diluting solution

WS:

Washing solution

HTF:

Human tubal fluid

rRNA:

Ribosome RNA

FPKM:

Fragment per kilobase of transcript per million mapped reads

FC:

Fold change

PCA:

Principal component analysis

DEGs:

Differentially expressed genes

APA:

Advanced paternal age

NAFLD:

Nonalcoholic fatty liver disease

ROS:

Reactive oxygen free radicals

EMT:

Epithelial-mesenchymal transition

HCC:

Hepatocellular carcinoma

ASICs:

Acid-sensing ion channels

LBP:

Lipopolysaccharide-binding protein

ICRs:

Imprinted control regions

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Acknowledgements

We thank MDPI (https://www.mdpi.com/authors/english) for its linguistic assistance during the preparation of this manuscript.

Funding

This work was supported by the National Natural Science Foundation of China (82001632), China Postdoctoral Science Foundation (2021M690929), and the Joint Construction Project of Henan Medical Science and Technology Project (SBGJ202001002).

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  1. Lei Zhang and Huanhuan Chen contributed equally to this work.

Authors and Affiliations

  1. Reproductive Medicine Center, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Henan Provincial People’s Hospital of Henan University, Zhengzhou, Henan, China

    Lei Zhang, Huanhuan Chen, Chenchen Cui, Linlin Liang, Hengtao Ge, Li Meng & Cuilian Zhang

  2. Henan Joint International Research Laboratory of Reproductive Bioengineering, Zhengzhou, Henan Province, China

    Lei Zhang, Huanhuan Chen, Chenchen Cui, Linlin Liang, Hengtao Ge, Li Meng & Cuilian Zhang

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Contributions

Conceptualization: Lei Zhang, Huanhuan Chen. Methodology: Chenchen Cui. Formal analysis and investigation: Lei Zhang, Hengtao Ge. Writing—original draft preparation: Lei Zhang. Writing—review and editing: Huanhuan Chen, Li Meng. Funding acquisition: Huanhuan Chen, Cuilian Zhang. Resources: Linlin Liang.

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Correspondence to Li Meng or Cuilian Zhang.

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Zhang, L., Chen, H., Cui, C. et al. Effects of oocyte vitrification on gene expression in the liver and kidney tissues of adult offspring. J Assist Reprod Genet 39, 2635–2646 (2022). https://doi.org/10.1007/s10815-022-02611-z

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