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Interaction between endometrial microbiota and host gene regulation in recurrent implantation failure

  • Reproductive physiology and disease
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Aim

To learn about the interaction between endometrial microbiota and host gene regulation in recurrent implantation failure.

Methods

The endometrial microbiota of 111 patients (RIF, 75; CON, 36) was analyzed by using 16 s rRNA sequencing technology. Transcriptome sequencing analysis of the endometrial of 60 patients was performed by using high-throughput sequencing.

Results

We found that the structure and composition of endometrium microbiota community of RIF patients were significantly different from those in control group. The abnormality of microbial structure and composition might interfere with the implantation of embryos by affecting the immune adaptation of the endometrium and the formation of endometrial blood vessels.

Conclusions

Our research described the host-microbe interaction in RIF. The structure and composition of endometrium microbiota community of RIF patients were significantly different from those in CON group. The abnormality of microbial structure and composition might interfere with the implantation of embryos by affecting the immune adaptation of the endometrium and the formation of endometrial blood vessels.

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Data availability

The 16 s rRNA gene sequencing for 75 endometrial microbiota samples have been deposited with the National Center for Biotechnology Information (NCBI) under reference number PRJNA732058. The transcriptome sequencing for 40 endometrium samples have been deposited with the NCBI under reference number PRJNA747622.

Abbreviations

RIF:

Recurrent implantation failure

ERA:

Endometrial receptivity array

WOI:

Window of implantation

FSH:

Follicle stimulation hormone

PICRUSt2:

Phylogenetic Investigation of Communities by Reconstruction of Unobserved States

LEfSe:

Linear discriminant analysis effect size

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Funding

National Natural Science Foundation of China [grant number 81871214] and National Key R&D Program of China [grant number 2017YFC1001603].

Author information

Authors and Affiliations

  1. Reproductive Medicine Center, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510655, China

    Peigen Chen, Lei Jia, Yi Zhou, Yingchun Guo, Cong Fang & Tingting Li

Authors
  1. Peigen Chen
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  2. Lei Jia
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  3. Yi Zhou
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  4. Yingchun Guo
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  5. Cong Fang
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  6. Tingting Li
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Corresponding authors

Correspondence to Cong Fang or Tingting Li.

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Ethics approval and consent to participate

The Ethics Committee approved the study of Sixth Affiliated Hospital of Sun Yat-Sen University (L2021ZSLYEC-280).

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Not applicable.

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The authors declare no competing interests.

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Supplementary Information

Below is the link to the electronic supplementary material.

Table S1

Clinical features of subject. (XLSX 17 kb)

Table S2

Differences in genus of RIF patients with different clinical characteristics. (XLSX 632 kb)

Supplement material 1 (PDF 138 kb)

Figure S1

(A) The rarefaction curve of 16s rRNA data. The composition of Phylum (B) and Genus (C) of inter-individual in each group. (PNG 352 kb)

High resolution image (TIF 1731 kb)

Figure S2

The co-occurrence network of RIF group (A) and CON group (B) based on SparCC. (PNG 473 kb)

High resolution image (TIF 750 kb)

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Chen, P., Jia, L., Zhou, Y. et al. Interaction between endometrial microbiota and host gene regulation in recurrent implantation failure. J Assist Reprod Genet 39, 2169–2178 (2022). https://doi.org/10.1007/s10815-022-02573-2

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  • DOI: https://doi.org/10.1007/s10815-022-02573-2

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