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A case series to examine the perinatal outcomes of infants conceived by intravaginal culture (IVC)

Abstract

Purpose

In vitro fertilization (IVF) has been a well-established method for treating infertility for over four decades. The mainstay method of culture of oocytes and embryos has been in gas incubators. More recently, the novel use of a gas-permeable closed vessel to culture oocytes and embryos in the vagina, intravaginal culture (IVC), has been introduced as a viable lower-cost option for infertility patients. Several studies have studied the efficacy of IVC; however, there is no data on the perinatal outcomes of the babies born using this newer technology.

Methods

Our study is a retrospective case series (n = 66) from a single center, uniquely examining the perinatal outcomes of infants born after IVC.

Results

There were 50 singleton and 16 twin gestations in this case series. For singleton infants conceived via IVC (n = 50), the mean gestational age at delivery was 38 weeks and 4 days, and the mean birth weight was 3159.1 + / − 501.5 g. Four infants were born with low birth weight, three were born preterm, and one was born macrosomic. The twin pregnancies had a mean gestational age at delivery of 33 weeks 4 days and a mean birth weight of 1992.9 + / − 620.7 g. Twenty-seven infants met the criteria for low birthweight, and twenty-four infants delivered preterm. No twin infants met the criteria for macrosomia.

Conclusion

This case series provides an initial description of the perinatal outcomes of IVC conceived infants, which shows no concerning trends in adverse birth outcomes for singleton infants. As expected, IVC twin gestations had a high rate of low birth weight and preterm delivery. Continued larger studies are essential to provide more comprehensive data on perinatal outcomes of infants conceived by this new technology.

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References

  1. Ranoux C, et al. A new in vitro fertilization technique: intravaginal culture. Fertil Steril. 1988;49(4):654–7.

    Article  CAS  Google Scholar 

  2. Sterzik K, et al. A new variation of in-vitro fertilization: intravaginal culture of human oocytes and cleavage stages. Hum Reprod. 1989;4(8 Suppl):83–6.

    Article  CAS  Google Scholar 

  3. Lucena E, et al. INVO procedure: minimally invasive IVF as an alternative treatment option for infertile couples. Sci World J. 2012;2012: 571596.

  4. Mitri F, et al. A pilot study to evaluate a device for the intravaginal culture of embryos. Reprod Biomed Online. 2015;31(6):732–8.

    Article  Google Scholar 

  5. Doody KJ, Broome EJ, Doody KM. Comparing blastocyst quality and live birth rates of intravaginal culture using INVOcell™ to traditional in vitro incubation in a randomized open-label prospective controlled trial. J Assist Reprod Genet. 2016;33(4):495–500.

    Article  PubMed Central  Google Scholar 

  6. Maheshwari A, et al. Is frozen embryo transfer better for mothers and babies? Can cumulative meta-analysis provide a definitive answer? Hum Reprod Update. 2018;24(1):35–58.

    Article  Google Scholar 

  7. Shah JS, et al. Perinatal outcomes in singleton pregnancies after in vitro fertilization cycles over 24 years. Fertil Steril. 2021;116(1):27–35.

    Article  Google Scholar 

  8. Zhou F, et al. Perinatal outcomes in vanishing twin pregnancies following assisted reproductive technology (ART) - a systematic review and meta-analysis. J Perinat Med. 2020;48(7):639–47.

    Article  Google Scholar 

  9. Geisler ME, et al. Obstetric and perinatal outcomes of twin pregnancies conceived following IVF/ICSI treatment compared with spontaneously conceived twin pregnancies. Eur J Obstet Gynecol Reprod Biol. 2014;181:78–83.

    Article  Google Scholar 

  10. Stern JE, et al. Adverse pregnancy and birth outcomes associated with underlying diagnosis with and without assisted reproductive technology treatment. Fertil Steril. 2015;103(6):1438–45.

    Article  PubMed Central  Google Scholar 

  11. Shapiro BS, et al. Evidence of impaired endometrial receptivity after ovarian stimulation for in vitro fertilization: a prospective randomized trial comparing fresh and frozen-thawed embryo transfer in normal responders. Fertil Steril. 2011;96(2):344–8.

    Article  Google Scholar 

  12. Sullivan-Pyke CS, et al. In vitro fertilization and adverse obstetric and perinatal outcomes. Semin Perinatol. 2017;41(6):345–53.

    Article  PubMed Central  Google Scholar 

  13. Ishihara O, et al. Impact of frozen-thawed single-blastocyst transfer on maternal and neonatal outcome: an analysis of 277,042 single-embryo transfer cycles from 2008 to 2010 in Japan. Fertil Steril. 2014;101(1):128–33.

    Article  Google Scholar 

  14. Wennerholm UB, et al. Perinatal outcomes of children born after frozen-thawed embryo transfer: a Nordic cohort study from the CoNARTaS group. Hum Reprod. 2013;28(9):2545–53.

    Article  Google Scholar 

  15. Kamath MS, et al. Perinatal outcomes after stimulated versus natural cycle IVF: a systematic review and meta-analysis. Reprod Biomed Online. 2018;36(1):94–101.

    Article  Google Scholar 

  16. Mak W, et al. Natural cycle IVF reduces the risk of low birth weight infants compared with conventional stimulated IVF. Hum Reprod. 2016;31(4):789–94.

    Article  PubMed Central  Google Scholar 

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Correspondence to Winifred Mak.

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Conflict of interest

F.A. is a member of the medical advisory board for INVO Biosciences.

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Kaye, M., Williams, E., Anderson, A. et al. A case series to examine the perinatal outcomes of infants conceived by intravaginal culture (IVC). J Assist Reprod Genet 39, 1367–1371 (2022). https://doi.org/10.1007/s10815-022-02490-4

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  • DOI: https://doi.org/10.1007/s10815-022-02490-4

Keywords

  • Intravaginal culture
  • In vitro fertilization
  • Perinatal outcomes