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The function of Nucleoporin 37 on mouse oocyte maturation and preimplantation embryo development

  • Embryo Biology
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Abstract

Purpose

Nucleoporin 37 (NUP37) has been reported to activate the YAP-TEAD signaling, which is crucial for early embryo development. However, whether NUP37 is involved in oocyte meiosis and embryo development remains largely unknown. The study aimed to clarify the function of Nup37 in oocyte maturation and early embryo development, and to explore the mechanism.

Methods

The expression level and subcellular localization of NUP37 were explored. After knocking down of Nup37 by microinjecting interfering RNA (siRNA), the oocyte maturation rate, aberrant PB1 extrusion rate, and blastocyst formation rate were evaluated. In addition, the effect of the downregulation of Nup37 on YAP-TEAD signaling was confirmed by immunofluorescence staining and real-time quantitative PCR.

Results

NUP37 was highly expressed in oocytes and early embryos; it mainly localized to the nuclear periphery at mice GV stage oocytes and early embryos. Nup37 depletion led to aberrant PB1 extrusion at the MII stage oocyte and a decreased blastocyst formation rate. The reduction of NUP37 caused YAP1 mislocalization and decreased the expression of Tead1, Tead2, and Tead4 during mice embryo development, thus affecting the YAP-TEAD activity and embryo developmental competence.

Conclusions

In summary, NUP37 played an important role in mice oocyte maturation and preimplantation embryo development.

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Data availability

The data underlying this article is available in the article and in its online supplementary material.

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Acknowledgements

The authors would like to thank Jiaxi Cheng for her careful discussion on the content of the manuscript.

Funding

This work was supported by the National Key Research and Development Program of China (2017YFA0103801) and the National Natural Science Foundation of China (No. 31871447, No. 31571544, and No. 31522034).

Author information

Author notes

  1. Qianying Guo and Qiang Liu contributed equally to this work.

Authors and Affiliations

  1. Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China

    Qianying Guo, Qiang Liu, Nan Wang, Jing Wang, Andi Sun, Jie Qiao & Liying Yan

  2. National Clinical Research Center for Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China

    Qianying Guo, Qiang Liu, Nan Wang, Jing Wang, Andi Sun, Jie Qiao & Liying Yan

  3. Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education Beijing Key, Beijing, 100191, China

    Qianying Guo, Qiang Liu, Nan Wang, Jing Wang, Andi Sun, Jie Qiao & Liying Yan

  4. Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China

    Qianying Guo, Qiang Liu, Nan Wang, Jing Wang, Andi Sun, Jie Qiao & Liying Yan

  5. Research Units of Comprehensive Diagnosis and Treatment of Oocyte Maturation Arrest, Chinese Academy of Medical Sciences, Beijing, China

    Qianying Guo, Qiang Liu, Nan Wang, Jing Wang, Andi Sun, Jie Qiao & Liying Yan

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  1. Qianying Guo
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Contributions

Q.G., Q.L., N.W., and A.S. performed the experiments. Q.G. and J.W. analyzed the data. Q.G., Q.L., and L.Y. designed the study. Q.G., Q.L, and L.Y. wrote the manuscript with help from all authors. The manuscript has been approved by all authors for publication.

Corresponding author

Correspondence to Liying Yan.

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Ethics approval

Animal experimental procedures were followed as the Institutional Animal Welfare and Ethics Committee policies of Peking University (LA2018261).

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The authors declare no competing interests.

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Supplementary information

ESM 1

Subcellular localization of Nup37 at the 8-cell stage. Immunofluorescent staining of Nup37 showing that in some cases Nup37 localized at the nucleus at the 8-cell stage. Scale bar, 20μm. (PNG 3604 kb)

High resulotion image (TIF 2048 kb)

ESM 2

NUP37 inhibition did not affect chromosome ploidy. (A) Metaphase spread of chromosomes from SiNC and SiNup37 MII oocytes, chromosomes were stained with DAPI. Scale bar, 10μm. (B) The chromosome ploidy was recorded and compared between the SiNC (n = 24) and SiNup37 (n = 46) group. (C) There was no significant difference in euploidy rate between SiNC and SiNup37 (P = 0.603, Chi-Squared Test). (PNG 561 kb)

High resulotion image (TIF 380 kb)

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Guo, Q., Liu, Q., Wang, N. et al. The function of Nucleoporin 37 on mouse oocyte maturation and preimplantation embryo development. J Assist Reprod Genet 39, 107–116 (2022). https://doi.org/10.1007/s10815-021-02330-x

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  • DOI: https://doi.org/10.1007/s10815-021-02330-x

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