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Early rescue oocyte activation for activation-impaired oocytes with no second polar body extrusion after intracytoplasmic sperm injection

  • Assisted Reproduction Technologies
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Abstract

Purpose

When rescue artificial oocyte activation (ROA) is performed on the day after intracytoplasmic sperm injection (ICSI) or later, embryonic development is poor and seldom results in live births. The efficacy of an early ROA after ICSI is unclear. Is early ROA effective in rescuing unfertilized oocytes that have not undergone second polar body extrusion several hours after ICSI?

Methods

We performed retrospective cohort study between October 2016 and September 2019, targeting 2891 oocytes in 843 cycles when ICSI was performed. We performed ROA with calcium ionophore on 395 of the 475 oocytes with no second polar extrusion 2.5–6 h after ICSI.

Results

The normal fertilization rate of ROA oocytes was significantly higher than non-ROA oocytes (65.8% vs 6.7%, P < 0.001). The blastocyst development rate in ROA oocytes was significantly lower than spontaneously activated oocytes (48.9% vs 67.2%, P < 0.001). The ROA oocyte implantation rate did not significantly differ from the spontaneously activated oocytes (36.0% vs 41.2%). We observed no differences in the implantation rates and blastocyst development rates over the 2.5–6 h from ICSI until ROA.

Conclusion

Early ROA is effective, and the optimal timing appears to be 2.5–6 h after ICSI.

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Authors and Affiliations

  1. Minoura Ladies Clinic, 3-9-17 Isoyama, Suzuka, Mie, 510-0256, Japan

    Takashi Shibahara, Yuu Fukasaku, Naoko Hayashi, Nozomi Miyazaki & Hiroyuki Minoura

  2. Kawato Ladies Clinic, 1-16-11 Betumei, Yokkaichi, Mie, 510-0007, Japan

    Hiroaki Kawato

Authors
  1. Takashi Shibahara
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  2. Yuu Fukasaku
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  3. Naoko Hayashi
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  4. Nozomi Miyazaki
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  5. Hiroaki Kawato
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  6. Hiroyuki Minoura
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Yuu Fukasaku, Naoko Hayashi, Nozomi Miyazaki, Hiroaki Kawato, and Hiroyuki Minoura. The first draft of the manuscript was written by Takashi Shibahara, and all authors commented on the previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Takashi Shibahara.

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Ethics approval

Approval was obtained from the ethics committee of Minoura Ladies Clinic. The procedures used in this study adhere to the tenets of the Declaration of Helsinki.

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Informed consent was obtained verbally from all individual participants included in the study.

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The participants verbally consented to the submission to the journal.

Conflict of interest

The authors declare no competing interests.

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Shibahara, T., Fukasaku, Y., Hayashi, N. et al. Early rescue oocyte activation for activation-impaired oocytes with no second polar body extrusion after intracytoplasmic sperm injection. J Assist Reprod Genet 38, 1061–1068 (2021). https://doi.org/10.1007/s10815-021-02089-1

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  • DOI: https://doi.org/10.1007/s10815-021-02089-1

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