Conventional ICSI improves the euploid embryo rate in male reciprocal translocation carriers

Abstract

Purpose

To evaluate whether the morphologically normal spermatozoa selected for intracytoplasmic sperm injection (ICSI) under microscope had a higher rate of normal/balanced chromosome contents than that in the whole unselected sperm from reciprocal translocation carriers.

Methods

Five hundred unselected spermatozoa from each of 40 male translocation carriers were performed with fluorescence in situ hybridization (FISH), to determine the rates of gametes with different meiotic contents of translocated chromosomes. Meanwhile, 3030 biopsied blastocysts from 239 male and 293 female reciprocal translocation carriers were detected with the microarray technique to analyze the rates of embryos with different translocated chromosome contents.

Results

The D3 embryo rate, blastocyst formation rate, and euploid rate of blastocysts were remarkably higher in male carriers than those in female (p = 0.001, p = 0.004, and p = 0.035, respectively). In addition, the percentages of alternate products, which contained normal/balanced chromosome contents, in embryos from male carriers were markedly higher than those in sperm FISH (p = 2.48 × 10−5 and p = 2.88 × 10−10), while the percentages of adjacent-2 and 3:1 products were lower than those in sperm FISH (p = 0.003 and p = 5.28 × 10−44). Moreover, consistent results were obtained when comparing the rates of products in embryos between male and female carriers. Specifically, the incidence of alternate products in male carriers was higher than those in female carriers (p = 0.022). However, no similar differences were seen between sperm and embryos of female carriers.

Conclusion

ICSI facilitates the selection of spermatozoa with normal/balanced chromosome contents and improves the D3 embryo rate, blastocyst formation rate, and the euploid embryo rate in male carriers.

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Acknowledgments

We express deepest gratitude to all the families enrolled in our research. We also thank the staff of the Genetics Laboratory and IVF Laboratory for their invaluable efforts and contribution relating to the experiment.

Funding

The research was supported by the Science and Technology Innovation Action Plan Program of Shanghai (18411953800) and Shanghai Shen Kang Hospital Development Centre Municipal Hospital New Frontier Technology Joint Project (SHDC12017105).

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Authors

Contributions

C.L, S.Z, and X.S designed the research and wrote the manuscript; C.L, S.Z, SJ.Z, J.W, M.X, J.Z, J.F, Y.S X.S, and C.L executed the research and data collection (S.Z, M.X, C.L, and M.X performed the analysis; S.Z, SJ.Z, and J.Z performed the microarray experiments and SJ.Z performed the FISH experiments; J.F and Y.S performed the intra-cytoplasmic sperm injection and blastocyst biopsy experiments; J.W, X.S, and C.L collected the cases). X.S and C.C directed the critical discussion of the manuscript. All authors approved the final manuscript.

Corresponding author

Correspondence to Xiaoxi Sun.

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The study protocol was approved by the Ethics Committee of Human Subject Research of the Obstetrics and Gynecology Hospital, Fudan University.

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The authors declare that they have no conflict of interest.

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Informed consents were obtained from all individual participants included in the study.

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Lei, C., Zhang, S., Zhu, S. et al. Conventional ICSI improves the euploid embryo rate in male reciprocal translocation carriers. J Assist Reprod Genet 38, 129–138 (2021). https://doi.org/10.1007/s10815-020-02013-z

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Keywords

  • Male reciprocal translocation
  • Sperm FISH
  • ICSI
  • Quadrivalent structure
  • Segregation patterns
  • Alternate products