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The lipidome of endometrial fluid differs between implantative and non-implantative IVF cycles

Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Objective

To characterize the most relevant changes in the lipidome of endometrial fluid aspirate (EFA) in non-implantative cycles.

Design

Lipidomics in a prospective cohort study.

Settings

Reproductive unit of a university hospital.

Patients

Twenty-nine women undergoing an IVF cycle. Fifteen achieved pregnancy and 14 did not.

Intervention

Endometrial fluid aspiration immediately before performing embryo transfer.

Main outcome measures

Clinical pregnancy rate and lipidomic profiles obtained on an ultra-high performance liquid chromatography coupled to time-of-flight mass spectrometry (UHPLC-ToF-MS)-based analytical platform.

Results

The comparative analysis of the lipidomic patterns of endometrial fluid in implantative and non-implantative IVF cycles revealed eight altered metabolites: seven glycerophospholipids and an omega-6 polyunsaturated fatty acid. Then, women with a non-implantative cycle were accurately classified with a support vector machine algorithm including these eight lipid metabolites. The diagnostic performances of the algorithm showed an area under the receiver operating characteristic curve, sensitivity, specificity, and accuracy of 0.893 ± 0.07, 85.7%, 80.0%, and 82.8%, respectively.

Conclusion

A predictive lipidomic signature linked to the implantative status of the endometrial fluid has been found.

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Funding

This study was partially funded by a Grant for Fertility Innovation (GFI, 2011) from Merck (Darmstadt, Germany).

Author information

Authors and Affiliations

  1. Human Reproduction Unit, Cruces University Hospital, BioCruces, University of the Basque Country, Bilbao, Spain

    Roberto Matorras, Blanca Corral, Jone Ibañez-Perez, Antonia Exposito & Begoña Prieto

  2. Instituto Valenciano de Infertilidad, IVI, Bilbao, Spain

    Roberto Matorras & Begoña Prieto

  3. OWL Metabolomics, Parque Tecnológico de Bizkaia, Derio, Spain

    Ibon Martinez-Arranz, Enara Arretxe, Marta Iruarrizaga-Lejarreta & Cristina Alonso

  4. Proteomics Platform, CIC bioGUNE, CIBERehd, ProteoRed-ISCIII, Parque Tecnológico de Bizkaia, Derio, Spain

    Felix Elortza

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  1. Roberto Matorras
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Correspondence to Roberto Matorras.

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IM-A, EA, MI-L, and CA are OWL Metabolomics’ employees.

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Matorras, R., Martinez-Arranz, I., Arretxe, E. et al. The lipidome of endometrial fluid differs between implantative and non-implantative IVF cycles. J Assist Reprod Genet 37, 385–394 (2020). https://doi.org/10.1007/s10815-019-01670-z

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  • DOI: https://doi.org/10.1007/s10815-019-01670-z

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