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Interleukin-23 receptor (IL-23R) gene polymorphisms and haplotypes associated with the risk of preeclampsia: evidence from cross-sectional and in silico studies

  • Genetics
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Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Pre-eclampsia is a relatively common pregnancy disorder. Serum concentrations of certain pro-inflammatory molecules and cytokines like interleukin-23 may affect the pathogenesis of pre-eclampsia. The interleukin-23 receptor (IL-23R) gene plays an important role in the progression of inflammatory and autoimmune diseases and IL-23 polymorphisms might influence the susceptibility of pre-eclampsia. The aim of the recent study was to establish the association between IL-23R gene polymorphisms and the susceptibility for developing of pre-eclampsia.

Methods

One hundred and fifty-eight pregnant patients with pre-eclampsia and 153 controls were genotyped using RFLP-PCR and AS-PCR. Also, an in silico analysis was performed to predict possible effects of these variations on IL-23R mRNA and protein structures.

Results

The frequency of the AG genotype of rs11209026 is related to a higher risk of pre-eclampsia. The mutant C and A allele in rs10889677 and rs11209026 SNPs, respectively, are correlated with the risk of pre-eclampsia and they are more frequent in severe late onset PE. We found higher frequency of the haplotype CG in patients with pre-eclampsia in comparison to healthy controls, as well as, the CG haplotype frequency significantly increased the risk of PE in severe, early onset, and late onset sub-groups. The results of computational analysis predicted rs11209026 and rs10889677 SNPs as functional variations, which can influence IL-23R mRNA and protein.

Conclusions

The results of present study show positive association between polymorphisms in the IL-23R gene and pre-eclampsia. Therefore, the presence of IL-23R rs11209026, rs10889677 polymorphism might be markers for the genetic susceptibility to pre-eclampsia.

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Funding

This study supported by grant number 171/94 (Zbmu.1.REC.1396.25) from the Zabol University of Medical Sciences.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran

    Danial Jahantigh

  2. Department of Obstetrics and Gynecology, Faculty of Medicine, Zabol University of Medical Sciences, Zabol, Iran

    Forough Forghani

  3. Department of Cell and Molecular Biology, Kosar University of Bojnord, Bojnord, Iran

    Saeedeh Ghazaey Zidanloo

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  1. Danial Jahantigh
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  2. Forough Forghani
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  3. Saeedeh Ghazaey Zidanloo
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Correspondence to Forough Forghani.

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Jahantigh, D., Forghani, F. & Zidanloo, S.G. Interleukin-23 receptor (IL-23R) gene polymorphisms and haplotypes associated with the risk of preeclampsia: evidence from cross-sectional and in silico studies. J Assist Reprod Genet 36, 1523–1536 (2019). https://doi.org/10.1007/s10815-019-01479-w

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  • DOI: https://doi.org/10.1007/s10815-019-01479-w

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