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Enhancement of the efficiency of oocyte vitrification through regulation of histone deacetylase 6 expression

Journal of Assisted Reproduction and Genetics volume 35, pages 1179–1185 (2018)Cite this article

Abstract

Objective

Successful oocyte vitrification (OV) is critical for cryopreservation of the oocytes from female patients with infertility, polycystic ovaries, and gynecologic cancers. Recent evidence suggests that relatively low levels of histone acetylation are critical for maintenance of the maturation capacity of cryopreserved oocytes. However, previous studies have only demonstrated a key role of histone deacetylases (HDAC) 1 and 2 in the cryopreservation of oocytes.

Methods

In this study, we investigated the role of HDAC6 in these settings. We found that mouse oocytes with low HDAC6 levels decreased survival rate, cleavage rate, and blastocyst rate after OV. Bioinformatics analyses were used to predict HDAC6-targeting microRNAs (miRNAs), while the functional binding of miRNAs to HDAC6 mRNA was evaluated by a dual luciferase reporter assay.

Results

Among all HDAC6-targeting miRNAs, we detected expression of miR-558, miR-527, and miR-762 in mouse oocytes. Specifically, we found that only miR-762 significantly inhibited protein translation of HDAC6 via binding to the 3′-UTR of the HDAC6 mRNA. Transfection of oocytes with HDAC6 or antisense of miR-762 significantly increased the survival rate, the cleavage rate, and blastocyst rate after OV.

Conclusion

As a result, our data suggest that induction of HDAC6 levels by miR-762 suppression may improve the current protocol for OV.

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Funding

The research was supported by the Chinese National Natural Science foundation (81401263), Shanghai municipal health and Family Planning Commission of traditional Chinese medicine research (2014LP010A), Research project of Shanghai municipal health and Family Planning Commission (140826110504864), and Shanghai Jiao University Scientific and Technological Innovation Funds (17JCYA01).

Author information

Author notes

  1. Yan-ping Kuang and Song-guo Xue contributed equally to this work.

Affiliations

  1. Department of Assisted Reproduction, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhi Zao Ju Road, Shanghai, 200011, China

    Yao Wang, Lei-wen Zhao, Yan-ping Kuang & Song-guo Xue

  2. Center of Reproductive Medicine, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, 200135, China

    Yao Wang & Mei-lin Zhang

  3. Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200135, China

    Mei-lin Zhang

Authors
  1. Yao Wang
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  2. Mei-lin Zhang
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  3. Lei-wen Zhao
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  4. Yan-ping Kuang
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  5. Song-guo Xue
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Corresponding author

Correspondence to Song-guo Xue.

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The authors have declared that no competing interests exist.

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Wang, Y., Zhang, Ml., Zhao, Lw. et al. Enhancement of the efficiency of oocyte vitrification through regulation of histone deacetylase 6 expression. J Assist Reprod Genet 35, 1179–1185 (2018). https://doi.org/10.1007/s10815-018-1221-6

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  • DOI: https://doi.org/10.1007/s10815-018-1221-6

Keywords

  • miR-337-3p
  • Histone deacetylases 6 (HDAC6)
  • Oocyte vitrification (OV)