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Journal of Assisted Reproduction and Genetics

, Volume 35, Issue 7, pp 1161–1168 | Cite as

A new, simple, automatic vitrification device: preliminary results with murine and bovine oocytes and embryos

  • Amir AravEmail author
  • Yehudit Natan
  • Dorit Kalo
  • Alisa Komsky-Elbaz
  • Zvika Roth
  • Paolo Emanuele Levi-Setti
  • Milton Leong
  • Pasquale Patrizio
Technological Innovations

Abstract

Purpose

This paper reports the use of a novel automatic vitrification device (Sarah, Fertilesafe, Israel) for cryopreservation of oocytes and embryos.

Methods

Mice oocytes (n = 40) and embryos (8 cells, n = 35 and blastocysts, n = 165), bovine embryos (2PN, n = 35), and MII oocytes (n = 84) were vitrified using this automated device. A total of 42 (2 cells) mice embryos, 20 (2PN) bovine embryos, and 150 MII bovine oocytes were used as fresh controls and grown to blastocysts. Upon rewarming, all were assessed for viability, cleavage, blastocyst, and hatching rates.

Results

Ninety-five % (38/40) of the mice MII oocytes regained isotonic volumes and all (100%) the surviving were viable. Rewarmed 8-cell mice embryos had 95% (33/35) blastulation rate and 80% (28/35) hatched. Rewarmed mice blastocysts had 97% survival rate (160/165) and 81% (135/165) hatched. Fresh control mice embryos had 100% (42/42) blastulation and 73% (21/42) hatching rates. Bovine embryos’ survival was 100% with 54% (19/35) cleavage and 9% (3/35) blastulation rate. Fresh control bovine embryos had 65% (13/20) cleavage and 20% (4/20) blastulation rate. Vitrified bovine oocytes had 100% survival (84/84), 73% (61/84) cleavage, and 7% (6/84) blastocysts’ rates; fresh control had 83% (125/150) cleavage and 11% (17/150) blastocysts’ rates.

Conclusion

This novel automatic vitrification device is capable to produce high survival rates of oocytes and embryos. We anticipate that as the demand for vitrification of gametes, embryos, and reproductive tissues increases worldwide, the availability of an automated vitrification device will become indispensable for standardization, simplification, and reproducibility of the entire process.

Keywords

Vitrification Oocytes Embryos Automation Cryopreservation 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Amir Arav
    • 1
    Email author
  • Yehudit Natan
    • 1
  • Dorit Kalo
    • 2
    • 3
  • Alisa Komsky-Elbaz
    • 2
    • 3
  • Zvika Roth
    • 2
    • 3
  • Paolo Emanuele Levi-Setti
    • 4
  • Milton Leong
    • 5
  • Pasquale Patrizio
    • 1
    • 6
  1. 1.FertileSafe LtdNes-ZionaIsrael
  2. 2.Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and EnvironmentThe Hebrew UniversityRehovotIsrael
  3. 3.Center of Excellence in Agriculture and Environmental HealthThe Hebrew UniversityRehovotIsrael
  4. 4.Department of Gynecology, Division of Gynecology and Reproductive Medicine, Humanitas Fertility CenterHumanitas Research HospitalMilanItaly
  5. 5.The Women’s ClinicHong KongHong Kong
  6. 6.Yale Fertility CenterNew HavenUSA

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