Outcome of immature oocytes collection of 119 cancer patients during ovarian tissue harvesting for fertility preservation
- 81 Downloads
Few clinical options for fertility preservation are available to females with cancer, and data about clinical outcomes is limited. Potential supplementary approaches to fertility preservation include retrieval of immature oocytes followed by in vitro maturation (IVM) and storage. The aim of this study was to evaluate post-thawing outcomes of immature oocytes collected both by transvaginal aspiration and from excised ovarian tissue.
We conducted a retrospective cohort study of patients treated in a single tertiary center. We reviewed the records of 119 cancer patients who underwent ovarian tissue cryopreservation and immature oocyte harvesting for fertility preservation. All embryos and oocytes that were frozen and thawed were included in the study. Post-thawing outcomes were evaluated.
Thirty-five stored embryos from eight patients were thawed. Twenty-nine embryos survived (82% survival rate) and were transferred. Six oocytes were thawed, two oocytes survived, and no oocytes were fertilized. Only one PCOS patient became pregnant, resulting in the normal delivery of a healthy baby.
Although a relatively high number of mature oocytes and embryos can be stored with the combined procedure, the limited rate of pregnancies represents a poor reproductive outcome. Therefore, this approach should be reserved for special groups with limited options.
KeywordsFertility preservation Oocyte retrieval In vitro oocyte maturation
Availability of data and materials
The datasets used and/or analyzed during the current study available from the corresponding author on reasonable request.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. For this type of study, formal consent is not required.
Conflict of interest
The authors declare that they have no competing interests.
- 4.Barton SE, Najita JS, Ginsburg ES, Leisenring WM, Stovall M, Weathers RE, et al. Infertility, infertility treatment, and achievement of pregnancy in female survivors of childhood cancer: a report from the childhood cancer survivor study cohort. Lancet Oncol. 2013;14(9):873–81. https://doi.org/10.1016/s1470-2045(13)70251-1.CrossRefPubMedGoogle Scholar
- 5.Society for Assisted Reproductive Technology, 2014 Clinic summary report. 2014. https://www.sartcorsonline.com/rptCSR_PublicMultYear.aspx?ClinicPKID=0. Accessed 14/09/2017 2017.
- 11.Donnez J, Dolmans MM, Pellicer A, Diaz-Garcia C, Sanchez Serrano M, Schmidt KT, et al. Restoration of ovarian activity and pregnancy after transplantation of cryopreserved ovarian tissue: a review of 60 cases of reimplantation. Fertil Steril. 2013;99(6):1503–13. https://doi.org/10.1016/j.fertnstert.2013.03.030.CrossRefPubMedGoogle Scholar
- 13.Martinez F. Update on fertility preservation from the Barcelona International Society for Fertility Preservation-ESHRE-ASRM 2015 expert meeting: indications, results and future perspectives. Fertil Steril. 2017;108(3):407–15.e11. https://doi.org/10.1016/j.fertnstert.2017.05.024.CrossRefPubMedGoogle Scholar
- 16.Hourvitz A, Yerushalmi GM, Maman E, Raanani H, Elizur S, Brengauz M, et al. Combination of ovarian tissue harvesting and immature oocyte collection for fertility preservation increases preservation yield. Reprod BioMed Online. 2015;31(4):497–505. https://doi.org/10.1016/j.rbmo.2015.06.025.CrossRefPubMedGoogle Scholar
- 18.Prasath EB, Chan ML, Wong WH, Lim CJ, Tharmalingam MD, Hendricks M, et al. First pregnancy and live birth resulting from cryopreserved embryos obtained from in vitro matured oocytes after oophorectomy in an ovarian cancer patient. Hum Reprod. 2014;29(2):276–8. https://doi.org/10.1093/humrep/det420.CrossRefPubMedGoogle Scholar
- 19.Segers I, Mateizel I, Van Moer E, Smitz J, Tournaye H, Verheyen G, et al. In vitro maturation (IVM) of oocytes recovered from ovariectomy specimens in the laboratory: a promising “ex vivo” method of oocyte cryopreservation resulting in the first report of an ongoing pregnancy in Europe. J Assist Reprod Genet. 2015;32(8):1221–31. https://doi.org/10.1007/s10815-015-0528-9.CrossRefPubMedPubMedCentralGoogle Scholar
- 20.Uzelac PS, Delaney AA, Christensen GL, Bohler HC, Nakajima ST. Live birth following in vitro maturation of oocytes retrieved from extracorporeal ovarian tissue aspiration and embryo cryopreservation for 5 years. Fertil Steril. 2015;104(5):1258–60. https://doi.org/10.1016/j.fertnstert.2015.07.1148.CrossRefPubMedGoogle Scholar
- 28.Yin H, Jiang H, Kristensen SG, Andersen CY. Vitrification of in vitro matured oocytes collected from surplus ovarian medulla tissue resulting from fertility preservation of ovarian cortex tissue. J Assist Reprod Genet. 2016;33(6):741–6. https://doi.org/10.1007/s10815-016-0691-7.CrossRefPubMedPubMedCentralGoogle Scholar
- 30.Sonigo C, Simon C, Boubaya M, Benoit A, Sifer C, Sermondade N, et al. What threshold values of antral follicle count and serum AMH levels should be considered for oocyte cryopreservation after in vitro maturation? Hum Reprod. 2016;31(7):1493–500. https://doi.org/10.1093/humrep/dew102.CrossRefPubMedGoogle Scholar