Re-analysis of aneuploidy blastocysts with an inner cell mass and different regional trophectoderm cells
- 388 Downloads
The purpose of this study is to explore which part of the trophectoderm best represents the inner cell mass after aCGH analysis.
Fifty-one preimplantation genetic diagnosis/preimplantation genetic screening of abnormal blastocysts diagnosed by array comparative genomic hybridization were included in this study. Blastocysts were thawed, incubated for 3 to 4 h, and then biopsied. Four regions were biopsied per blastocyst, including the inner cell mass (ICM), trophectoderm (TE) cells opposite the ICM, TE cells at the upper right of the ICM, and TE cells at the lower right of the ICM. The biopsied pieces were processed through multiple annealing and looping-based amplification cycle sequenced for 24-chromosome aneuploidy screening. The aneuploidy results were compared among the ICM and the different regional trophectoderm cells from the same blastocyst.
Fifty of 51 (98.04%) ICM samples were concordant with at least one of the TE biopsies derived from the same embryos. There were 43 blastocysts in which ICM and the other three TE pieces were consistent. Discordance among the four pieces occurred in eight blastocysts. Only one blastocyst was discordant between the ICM and the other three TE pieces, while seven blastocysts were discordant between one of TE and the other three biopsied pieces. There was no special region that the mosaic TE was located.
Our findings indicate that TE aneuploidy is an excellent predictor of ICM aneuploidy. The blastocyst mosaic cells are inclined to be located in TE. Moreover, the mosaic TE was not limited to the special region.
KeywordsBlastocyst 24-chromosome aneuploidy screening Multiple annealing and looping-based amplification cycle sequencing Mosaic Preimplantation genetic screening
Compliance with ethical standards
This study was approved by the Institutional Review Board of Peking University Third Hospital, China. Written informed consent was obtained from each couple.
This study was supported by the grants from National High Technology Research and Development Program (2015AA020407), Beijing Municipal Science and Technology Commission (Z131100005213006, CBXM2015-036), the National Natural Science of China (31522034), research fund of National Health and Family Planning Commission of China (201402004) and special funds of Guangxi-distinguished experts, China.
- 2.Yang Z, Liu J, Collins GS, Salem SA, Liu X, Lyle SS, et al. Selection of single blastocysts for fresh transfer via standard morphology assessment alone and with array CGH for good prognosis IVF patients: results from a randomized pilot study. Mol Cytogenet. 2012;5:24.CrossRefPubMedPubMedCentralGoogle Scholar
- 10.Schoolcraft WB, Treff NR, Stevens JM, Ferry K, Katz-Jaffe M, Scott Jr RT. Live birth outcome with trophectoderm biopsy, blastocyst vitrification, and single-nucleotide polymorphism microarray–based comprehensive chromosome screening in infertile patients. Fertil Steril. 2011;96:638–40.CrossRefPubMedGoogle Scholar
- 14.Chow JF, Yeung WS, Lau EY. Array comparative genomic hybridization analyses of all blastomeres of a cohort of embryos from young IVF patients revealed significant contribution of mitotic errors to embryo mosaicism at the cleavage stage. Reprod Biol Endocrinol. 2014;12:105.CrossRefPubMedPubMedCentralGoogle Scholar
- 19.Gardner DK, Schoolcraft WB. In vitro culture of human blastocysts. In: Jansen R, Mortimer D, editors. Towards reproductive certainty: infertility and genetics beyond. Carnforth, UK: Parthenon Press; 1999. p. 377–88.Google Scholar