Journal of Assisted Reproduction and Genetics

, Volume 34, Issue 4, pp 511–516 | Cite as

Adiponectin and leptin gene polymorphisms in women with gestational diabetes mellitus

  • Andrzej Pawlik
  • Joanna Teler
  • Agnieszka Maciejewska
  • Marek Sawczuk
  • Krzysztof Safranow
  • Violetta Dziedziejko



Gestational diabetes mellitus (GDM) is the glucose intolerance occurring during pregnancy. The prevalence of GDM is increased in obese women. Leptin and adiponectin are adipokines that play an important role in the regulation of insulin secretion and glucose and lipid metabolism. The aim of this study was to examine the association between adiponectin and leptin gene polymorphisms and the development of GDM.


This case–control study included 204 pregnant women with GDM and 207 pregnant women with normal glucose tolerance (NGT). The diagnosis of GDM was based on a 75-g oral glucose tolerance test (OGTT) at 24–28 weeks’ gestation. To discriminate the ADIPOQ rs266729, rs1501299 and LEP rs2167270 alleles, TaqMan® Pre-Designed SNP Genotyping Assays were used.


There was a statistically significant association between the ADIPOQ rs266729 gene polymorphism and GDM. Among women with GDM, a higher prevalence of the G allele was observed (GG and CG genotypes). Multivariate logistic regression analysis, taking into account age, BMI before pregnancy, past pregnancies and the ADIPOQ rs266729 gene polymorphism, revealed that the presence of a G allele is an independent risk factor for GDM. Moreover, there was the association between the LEP rs2167270 polymorphism and the requirement for daily insulin, which was significantly higher in women with the A allele (AA and GA genotypes).


The results of our study suggest an association between adiponectin gene rs266729 as well as leptin gene rs2167270 polymorphisms and GDM.


Gestational diabetes Adiponectin Leptin Polymorphism 


Compliance with ethical standards

The study was approved by the ethics committee in Pomeranian Medical University, Szczecin, Poland, and written informed consent was obtained from all subjects.

Conflict of interest

The authors declare that they have no conflict of interest.


  1. 1.
    Kim C. Gestational diabetes: risks, management, and treatment options. Int J Womens Health. 2010;2:339–51.CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Barbour LA, McCurdy CE, Hernandez TL, Kirwan JP, Catalano PM, Friedman JE. Cellular mechanisms for insulin resistance in normal pregnancy and gestational diabetes. Diabetes Care. 2007;30:112–9.CrossRefGoogle Scholar
  3. 3.
    Carbillon L, Boujenah J, Benbara A, Cosson E. Adverse outcomes and potential targets for intervention in gestational diabetes and obesity. Obstet Gynecol. 2015;126:1309–10.CrossRefPubMedGoogle Scholar
  4. 4.
    Sáinz N, Barrenetxe J, Moreno-Aliaga MJ, Martínez JA. Leptin resistance and diet-induced obesity: central and peripheral actions of leptin. Metabolism. 2015;64:35–46.CrossRefPubMedGoogle Scholar
  5. 5.
    Martin SS, Blaha MJ, Muse ED, Qasim AN, Reilly MP, Blumenthal RS, et al. Leptin and incident cardiovascular disease: the Multi-ethnic Study of Atherosclerosis (MESA). Atherosclerosis. 2015;239:67–72.CrossRefPubMedGoogle Scholar
  6. 6.
    Al-Azzam SI, Khabour OF, Alzoubi KH, Alzayadeen RN. The effect of leptin promoter and leptin receptor gene polymorphisms on lipid profile among the diabetic population: modulations by atorvastatin treatment and environmental factors. J Endocrinol Invest. 2014;37:835–42.CrossRefPubMedGoogle Scholar
  7. 7.
    Kadowaki T, Yamauchi T, Okada-Iwabu M, Iwabu M. Adiponectin and its receptors: implications for obesity-associated diseases and longevity. Lancet Diabetes Endocrinol. 2014;2:8–9.CrossRefPubMedGoogle Scholar
  8. 8.
    Jung CH, Kim BY, Mok JO, Kang SK, Kim CH. Association between serum adipocytokine levels and microangiopathies in patients with type 2 diabetes mellitus. J Diabetes Investig. 2014;5:333–9.CrossRefPubMedGoogle Scholar
  9. 9.
    Foucan L, Maimaitiming S, Larifla L, Hedreville S, Deloumeaux J, Joannes MO, et al. Adiponectin gene variants, adiponectin isoforms and cardiometabolic risk in type 2 diabetic patients. J Diabetes Investig. 2014;5:192–8.CrossRefPubMedGoogle Scholar
  10. 10.
    International Association of Diabetes and Pregnancy Study Groups Consensus Panel, Metzger BE, Gabbe SG, Persson B, Buchanan TA, Catalano PA, et al. International association of diabetes and pregnancy study groups recommendations on the diagnosis and classification of hyperglycemia in pregnancy. Diabetes Care. 2010;33:676–82.CrossRefPubMedCentralGoogle Scholar
  11. 11.
    Teler J, Tarnowski M, Safranow K, Maciejewska A, Sawczuk M, Dziedziejko V et al. CCL2, CCL5, IL4 and IL15 gene polymorphisms in women with gestational diabetes mellitus. Horm Metab Res. 2016, doi: 10.1055/s-0042-111436.
  12. 12.
    Lin CH, Ho CY, Liu CS, Lin WY, Li CI, Yang CW, et al. Influence of adiponectin gene polymorphisms on adiponectin serum level and insulin resistance index in taiwanese metabolic syndrome patients. Chin J Physiol. 2012;55:405–11.CrossRefPubMedGoogle Scholar
  13. 13.
    Petrone A, Zavarella S, Caiazzo A, Leto G, Spoletini M, Potenziani S, et al. The promoter region of the adiponectin gene is a determinant in modulating insulin sensitivity in childhood obesity. Obesity (Silver Spring). 2006;14:1498–504.CrossRefGoogle Scholar
  14. 14.
    Tankó LB, Siddiq A, Lecoeur C, Larsen PJ, Christiansen C, Walley A, et al. ACDC/adiponectin and PPAR-gamma gene polymorphisms: implications for features of obesity. Obes Res. 2005;13:2113–21.CrossRefPubMedGoogle Scholar
  15. 15.
    Gong M, Long J, Liu Q, Deng HC. Association of the ADIPOQ rs17360539 and rs266729 polymorphisms with type 2 diabetes: a meta-analysis. Mol Cell Endocrinol. 2010;325:78–83.CrossRefPubMedGoogle Scholar
  16. 16.
    Sahin DS, Tumer C, Demir C, Celik MM, Celik M, Ucar E, et al. Association with leptin gene C.-2548 G > A polymorphism, serum leptin levels, and body mass index in Turkish obese patients. Cell Biochem Biophys. 2013;65:243–7.CrossRefPubMedGoogle Scholar
  17. 17.
    Mammès O, Betoulle D, Aubert R, Herbeth B, Siest G, Fumeron F. Association of the G-2548A polymorphism in the 5′ region of the LEP gene with overweight. Ann Hum Genet. 2000;64:391–4.CrossRefPubMedGoogle Scholar
  18. 18.
    Retnakaran R, Hanley AJ, Raif N, Hirning CR, Connelly PW, Sermer M, et al. Adiponectin and beta cell dysfunction in gestational diabetes: pathophysiological implications. Diabetologia. 2005;48:993–1001.CrossRefPubMedGoogle Scholar
  19. 19.
    Desoye G, Hauguel-de MS. The human placenta in gestational diabetes mellitus. The insulin and cytokine network. Diabetes Care. 2007;30:120–6.CrossRefGoogle Scholar
  20. 20.
    Caminos JE, Nogueiras R, Gallego R, Bravo S, Tovar S, García-Caballero T, et al. Expression and regulation of adiponectin and receptor in human and rat placenta. J Clin Endocrinol Metab. 2005;90:4276–86.CrossRefPubMedGoogle Scholar
  21. 21.
    Doruk M, Uğur M, Oruç AS, Demirel N, Yildiz Y. Serum adiponectin in gestational diabetes and its relation to pregnancy outcome. J Obstet Gynaecol. 2014;34:471–5.CrossRefPubMedGoogle Scholar
  22. 22.
    Catalano PM, Hoegh M, Minium J, Huston-Presley L, Bernard S, Kalhan S, et al. Adiponectin in human pregnancy: implications for regulation of glucose and lipid metabolism. Diabetologia. 2006;49:1677–85.CrossRefPubMedGoogle Scholar
  23. 23.
    Hara K, Yamauchi T, Kadowaki T. Adiponectin: an adipokine linking adipocytes and type 2 diabetes in humans. Curr Diab Rep. 2005;5:136–40.CrossRefPubMedGoogle Scholar
  24. 24.
    Lam NT, Cheung AT, Riedel MJ, Light PE, Cheeseman CI, Kieffer TJ. Leptin reduces glucose transport and cellular ATP levels in INS-1 beta-cells. J Mol Endocrinol. 2004;32:415–24.CrossRefPubMedGoogle Scholar
  25. 25.
    Lee JW, Swick AG, Romsos DR. Leptin constrains phospholipase C-protein kinase C-induced insulin secretion via a phosphatidylinositol 3-kinase-dependent pathway. Exp Biol Med (Maywood). 2003;228:175–82.CrossRefGoogle Scholar
  26. 26.
    Soheilykhah S, Mojibian M, Rahimi-Saghand S, Rashidi M, Hadinedoushan H. Maternal serum leptin concentration in gestational diabetes. Taiwan J Obstet Gynecol. 2011;50:149–53.CrossRefPubMedGoogle Scholar
  27. 27.
    Pérez-Pérez A, Guadix P, Maymó J, Dueñas JL, Varone C, Fernández-Sánchez M, et al. Insulin and leptin signaling in placenta from gestational diabetic subjects. Horm Metab Res. 2016;48:62–9.PubMedGoogle Scholar
  28. 28.
    Beltcheva O, Boyadzhieva M, Angelova O, Mitev V, Kaneva R, Atanasova I. The rs266729 single-nucleotide polymorphism in the adiponectin gene shows association with gestational diabetes. Arch Gynecol Obstet. 2014;289:743–8.CrossRefPubMedGoogle Scholar
  29. 29.
    Han Y, Zheng YL, Fan YP, Liu MH, Lu XY, Tao Q. Association of adiponectin gene polymorphism 45TG with gestational diabetes mellitus diagnosed on the new IADPSG criteria, plasma adiponectin levels and adverse pregnancy outcomes. Clin Exp Med. 2015;15:47–53.CrossRefPubMedGoogle Scholar
  30. 30.
    Takhshid MA, Haem Z, Aboualizadeh F. The association of circulating adiponectin and +45 T/G polymorphism of adiponectin gene with gestational diabetes mellitus in Iranian population. J Diabetes Metab Disord. 2015;14:30.Google Scholar
  31. 31.
    Low CF, Mohd Tohit ER, Chong PP, Idris F. Adiponectin SNP45TG is associated with gestational diabetes mellitus. Arch Gynecol Obstet. 2011;283:1255–60.CrossRefPubMedGoogle Scholar
  32. 32.
    Vaskú JA, Vaskú A, Dostálová Z, Bienert P. Association of leptin genetic polymorphism −2548 G/A with gestational diabetes mellitus. Genes Nutr. 2006;1:117–23.CrossRefPubMedPubMedCentralGoogle Scholar
  33. 33.
    Hoffstedt J, Eriksson P, Mottagui-Tabar S, Arner P. A polymorphism in the leptin promoter region (−2548 G/A) influences gene expression and adipose tissue secretion of leptin. Horm Metab Res. 2002;34:355–9.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Andrzej Pawlik
    • 1
  • Joanna Teler
    • 2
  • Agnieszka Maciejewska
    • 3
  • Marek Sawczuk
    • 3
  • Krzysztof Safranow
    • 4
  • Violetta Dziedziejko
    • 4
  1. 1.Department of PhysiologyPomeranian Medical UniversitySzczecinPoland
  2. 2.Department of Obstetrics and GynecologyCounty HospitalZielona GoraPoland
  3. 3.Faculty of Physical Education and Health PromotionUniversity of SzczecinSzczecinPoland
  4. 4.Department of Biochemistry and Medical ChemistryPomeranian Medical UniversitySzczecinPoland

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