Journal of Assisted Reproduction and Genetics

, Volume 33, Issue 4, pp 473–480 | Cite as

Effect of cryopreservation on the properties of human endometrial stromal cells used in embryo co-culture systems

  • Ivan Bochev
  • Kalina Belemezova
  • Atanas Shterev
  • Stanimir Kyurkchiev
Embryo Biology
  • 171 Downloads

Abstract

Purpose

Along with comparative investigation of the decidualization potential and IL-6 secretion by fresh and frozen ESCs, we also aimed to evaluate the effectiveness of co-culture systems based on fresh or frozen ESCs in terms of clinical pregnancy rates.

Methods

Outcome analysis of a total of 215 IVF cycles with co-culture with fresh or frozen ESCs was performed. Endometrial tissue was obtained from 17 healthy donors. Concentrations of secreted prolactin, IGFBP-1, and IL-6 in conditioned media from cultured fresh and frozen ESCs (decidualized or not) were measured using ELISA or ECLIA.

Results

Embryo co-culture with frozen ESCs resulted in a much lower pregnancy rate compared to the alternative system using fresh ESCs. Furthermore, cultivated frozen ESCs showed considerably decreased release of prolactin, IGFBP-1, and IL-6 compared to fresh ESCs, indicating that cryopreservation negatively affects their decidualization potential and cytokine production.

Conclusions

Altogether, this data illustrates the need for optimization and improvement of the existing autologous endometrial co-culture systems.

Keywords

Embryo co-culture Endometrial stromal cells Cryopreservation Decidualization Cytokine production Pregnancy rate 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Ivan Bochev
    • 1
  • Kalina Belemezova
    • 2
  • Atanas Shterev
    • 1
  • Stanimir Kyurkchiev
    • 2
    • 3
  1. 1.IVF DepartmentOb/Gyn Hospital Dr. ShterevSofiaBulgaria
  2. 2.Tissue bank BULGENSofiaBulgaria
  3. 3.Institute of Reproductive HealthSofiaBulgaria

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