Effect of cryopreservation on the properties of human endometrial stromal cells used in embryo co-culture systems
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Along with comparative investigation of the decidualization potential and IL-6 secretion by fresh and frozen ESCs, we also aimed to evaluate the effectiveness of co-culture systems based on fresh or frozen ESCs in terms of clinical pregnancy rates.
Outcome analysis of a total of 215 IVF cycles with co-culture with fresh or frozen ESCs was performed. Endometrial tissue was obtained from 17 healthy donors. Concentrations of secreted prolactin, IGFBP-1, and IL-6 in conditioned media from cultured fresh and frozen ESCs (decidualized or not) were measured using ELISA or ECLIA.
Embryo co-culture with frozen ESCs resulted in a much lower pregnancy rate compared to the alternative system using fresh ESCs. Furthermore, cultivated frozen ESCs showed considerably decreased release of prolactin, IGFBP-1, and IL-6 compared to fresh ESCs, indicating that cryopreservation negatively affects their decidualization potential and cytokine production.
Altogether, this data illustrates the need for optimization and improvement of the existing autologous endometrial co-culture systems.
KeywordsEmbryo co-culture Endometrial stromal cells Cryopreservation Decidualization Cytokine production Pregnancy rate
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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