Age-associated alterations in the micromechanical properties of chromosomes in the mammalian egg
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The incidence of aneuploidy in eggs from women of advanced reproductive age can exceed 60 %, making the mammalian egg a unique model system to study the mechanisms of chromosome segregation errors.
Here we applied a novel biophysical chromosome stretching approach to quantify mechanical stiffness of meiotic chromosomes in the mammalian egg and then documented how these properties changed in a mouse model of physiologic reproductive aging.
We found significant differences in chromosome micromechanics, and thus in higher order chromosome structure, coincident with advanced reproductive age, a time that is also unequivocally associated with an increase in egg aneuploidy.
These findings have important implications for both reproductive and cancer biology where aneuploidy plays a central role in aging related disease states.
KeywordsReproductive aging Meiosis Mammalian Chromosome Micromechanics
This work was supported by grants from the National Institutes of Health (U54HD041857 to TKW and U54HD076188, U54CA143869 and R01GM105847 to JFM) and the National Science Foundation (NSF) (MCB-1022117 and DMR-1206868 to JFM).
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