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Reduced uterine receptivity for mouse embryos developed from in-vitro matured oocytes

  • Embryo Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The outcomes of in-vitro maturation (IVM) are inferior compared to those of IVF. The purpose of the study was to compare the implantation rates of IVM- and in-vivo maturation (IVO)- derived embryos, and to evaluate their effects on uterine receptivity.

Methods

The IVM- and IVO- oocytes were obtained from female mice, fertilized and transferred to separate oviducts of the same pseudo-pregnant mice. After 5 days, the implanted blastocysts were dissected out of the uterine horns, and the uterine horns were analyzed for the expression of mRNAs encoding leukemia inhibitory factor, heparin-binding epidermal growth factor, insulin-like growth factor binding protein-4, progesterone receptor, and Hoxa-10.

Results

The maturation rate of the IVM- oocytes was 81.2 %. The fertilization rate of the IVM oocytes was lower than that of the IVO oocytes (50.5 % vs. 78.0 %, p = 0.038), as was their implantation rate (14.5 % vs. 74.7 %, p < 0.001). All 5 mRNAs examined were expressed at significantly lower levels in the uterine horns that received the IVM-derived embryos than in those that received the IVO-derived embryos.

Conclusions

The IVM-derived embryos are less competent in inducing expression of implantation-related mRNAs in the uterine horn.

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Acknowledgments

This study was supported by the Shin Kong Wu Ho-Su Memorial Hospital (SKH-8302-100-DR-08).

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Shin Kong Wu Ho-Su Memorial Hospital, No. 95, Wen Chang Road, Shih Lin District, Taipei, Taiwan

    Yu-Hung Lin, Lee-Wen Huang, Kok-Min Seow & Jiann-Loung Hwang

  2. School of Medicine, Fu-Jen Catholic University, New Taipei City, Taiwan

    Yu-Hung Lin & Lee-Wen Huang

  3. Department of Obstetrics and Gynecology, National Taiwan University Hospital, Taipei, Taiwan

    Yu-Hung Lin

  4. Department of Obstetrics and Gynecology, Taipei Medical University, Taipei, Taiwan

    Yu-Hung Lin, Jiann-Loung Hwang & Chii-Ruey Tzeng

  5. Transgenic Core Facility, Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan

    Ching-Yen Tsai

  6. School of Medicine, National Yang-Ming University, Taipei, Taiwan

    Kok-Min Seow

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  1. Yu-Hung Lin
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Correspondence to Jiann-Loung Hwang.

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Capsule Embryos derived from in-vitro matured oocytes are less competent in inducing uterine receptivity.

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Lin, YH., Tsai, CY., Huang, LW. et al. Reduced uterine receptivity for mouse embryos developed from in-vitro matured oocytes. J Assist Reprod Genet 31, 1713–1718 (2014). https://doi.org/10.1007/s10815-014-0354-5

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  • DOI: https://doi.org/10.1007/s10815-014-0354-5

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