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The haplotype M2 of the ANXA5 gene is not associated with antitrophoblast antibodies

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The M2 haplotype in ANXA5 as well as antitrophoblast antibodies predispose to recurrent pregnancy loss (RPL). Since M2/ANXA5 can be a factor for development of antiphospholipid antibodies (aPL), this study aimed to trace a possible association of M2 with antitrophoblast antibodies.

Methods

One hundred patients with two or more consecutive, idiopathic RPLs were divided in two subgroups, JEG-3+ (n = 42) and JEG-3− (n = 58), according to the anti-JEG-3 reactivity measured in subjects’ sera. Both subgroups were genotyped for ANXA5 promoter haplotypes and genetic frequencies were compared to available fertile and control populations, as well as within the subgroups.

Results

M2/ANXA5 was generally enriched in the JEG-3 screened cohort of RPL patients in comparison to fertile and population controls. Despite the relatively higher abundance of the haplotype in the JEG-3− sample as compared to JEG-3+ patients and in the JEG-3− primary RPL subset in particular, compared to the rest of patients, there was no statistically significant difference between both, JEG-3− and JEG-3+ subgroups.

Conclusion

It appears that the haplotype M2/ANXA5 is not associated with the presence of anti-trophoblast antibodies. Our finding indicates that anti-trophoblast antibodies are a class of molecules that differ from aPL and from anti-b2-GPI antibodies, apparently not directed to same or similar epitopes that aPL and anti-b2-GPI would recognize.

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Acknowledgments

The authors wish to acknowledge the continuous support of IZKF Muenster for this study.

Conflict of interest

The Authors wish to declare no conflict of interest.

Author information

Authors and Affiliations

  1. Division of Gynecological Endocrinology and Reproductive Medicine, Department of Gynecology and Obstetrics, Klinikum der Ludwig-Maximilians-Universität, Munich, Campus Grosshadern, 81377, Munich, Germany

    Nina Rogenhofer, Laura Engels & Christian J. Thaler

  2. Insititute of Human Genetics, UKM and WWU Muenster, 48149, Muenster, Germany

    Nadja Bogdanova & Frank Tüttelmann

  3. Institute of Medical Biochemistry, ZMBE and IZKF, WWU Muenster, Von Esmarch Str. 56, 48149, Muenster, Germany

    Arseni Markoff

Authors
  1. Nina Rogenhofer
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  2. Laura Engels
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  3. Nadja Bogdanova
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  4. Frank Tüttelmann
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  5. Arseni Markoff
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  6. Christian J. Thaler
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Corresponding author

Correspondence to Arseni Markoff.

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Capsule

M2/ANXA5 carriage appears enriched in a subgroup of RPL patients with primary recurrent miscarriage and negative for antitrophoblast antibodies that is indicative of the haplotype's role as independent risk factor although the observed difference is not statistically significant.

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Rogenhofer, N., Engels, L., Bogdanova, N. et al. The haplotype M2 of the ANXA5 gene is not associated with antitrophoblast antibodies. J Assist Reprod Genet 30, 711–716 (2013). https://doi.org/10.1007/s10815-013-9978-0

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  • DOI: https://doi.org/10.1007/s10815-013-9978-0

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