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Long-term treatment of hydrogen-rich saline abates testicular oxidative stress induced by nicotine in mice

Abstract

Purpose

The present study was designed to test the hypothesis that long-term treatment with hydrogen-rich saline abated testicular oxidative stress induced by nicotine in mice.

Methods

The effects of hydrogen-rich saline (6 ml/kg, i.p.), vitamin C (60 mg/kg, i.p.) and vitamin E (100 mg/kg, i.p.) on reproductive system and testicular oxidative levels in nicotine-treated (4.5 mg/kg, s.b.) mice were investigated.

Results

It was found that vitamin C and vitamin E attenuated serum oxidative level, but did not lower testicular oxidative levels in mice subjected to chronic nicotine treatment, and did not improve the male reproductive damage and apoptosis induced by nicotine. Different from normal antioxidants, vitamin C and vitamin E, hydrogen-rich saline abated oxidative stress in testis, and protected against nicotine-induced male reproductive damages.

Conclusion

Our results first demonstrated that long-term treatment with hydrogen-rich saline attenuated testicular oxidative level and improved male reproductive function in nicotine-treated mice.

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Correspondence to Yi Zhang.

Additional information

Capsule Long-term treatment with hydrogen-rich saline could attenuate testicular oxidative level and improve male reproductive function in nicotine-treated mice.

Shu Li and DanDan Lu contribute equally to this work. Both persons are the first authors.

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Li, S., Lu, D., Zhang, Y. et al. Long-term treatment of hydrogen-rich saline abates testicular oxidative stress induced by nicotine in mice. J Assist Reprod Genet 31, 109–114 (2014). https://doi.org/10.1007/s10815-013-0102-2

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Keywords

  • Hydrogen-rich saline
  • Vitamin C
  • Vitamin E
  • Male reproductive system
  • Oxidative stress
  • Apoptosis