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Effect of micro-vibration culture system on embryo development

  • Embryo Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Micro-vibration culture system was examined to determine the effects on mouse and human embryo development and possible improvement of clinical outcomes in poor responders.

Materials and methods

The embryonic development rates and cell numbers of blastocysts were compared between a static culture group (n = 178) and a micro-vibration culture group (n = 181) in mice. The embryonic development rates and clinical results were compared between a static culture group (n = 159 cycles) and a micro-vibration culture group (n = 166 cycles) in poor responders. A micro-vibrator was set at a frequency of 42 Hz, 5 s/60 min duration for mouse and human embryo development.

Results

The embryonic development rate was significantly improved in the micro-vibration culture group in mice (p < 0.05). The cell numbers of mouse blastocysts were significantly higher in the micro-vibration group than in the static culture group (p < 0.05). In the poor responders, the rate of high grade embryos was not significantly improved in the micro-vibration culture group on day 3. However, the optimal embryonic development rate on day 5 was improved in the micro-vibration group, and the total pregnancy rate and implantation rate were significantly higher in the micro-vibration group than in the static culture group (p < 0.05).

Conclusions

Micro-vibration culture methods have a beneficial effect on embryonic development in mouse embryos. In poor responders, the embryo development rate was improved to a limited extent under the micro-vibration culture conditions, but the clinical results were significantly improved.

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Authors and Affiliations

  1. Department of Anatomy, School of Medicine, Kangwon National University, Chuncheon, 200-701, Korea

    Yong Soo Hur & Jeong Hyun Park

  2. Maria Fertility Hospital, 103-11 Sinseol-dong, Dongdaemun-gu, Seoul, 130-812, Korea

    Yong Soo Hur, Jeong Hyun Park, Eun Kyung Ryu, Sung Jin Park, Jun Ho Lee, Soo Hee Lee, Chang Young Hur, Won Don Lee & Jin Ho Lim

  3. Maria Research Center, 103-11, Sinseol-dong, Dongdaemun-gu, Seoul, 130-812, Korea

    Jung Yoon & San Hyun Yoon

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  1. Yong Soo Hur
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Correspondence to Yong Soo Hur.

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Capsule Micro-vibration culture system can improve mouse embryo development rate and clinical outcomes in poor responders.

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Hur, Y.S., Park, J.H., Ryu, E.K. et al. Effect of micro-vibration culture system on embryo development. J Assist Reprod Genet 30, 835–841 (2013). https://doi.org/10.1007/s10815-013-0007-0

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