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Germ cell abnormalities in streptozotocin induced diabetic mice do not correlate with blood glucose level

  • Gonadal Physiology and Disease
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To assess the effect of streptozotocin induced hyperglycemia on germ cell integrity, DNA ploidy and methylation status for a period of two spermatogenesis cycles in adult male Swiss albino mice.

Methods

Streptozotocin injected mice were monitored for hyperglycemia at a regular interval for a period of 36 and 72 days. The DNA integrity in epididymal spermatozoa was determined by the comet assay. Flow cytometric analysis was done in germ cells to assess the DNA ploidy. The global methylation analysis in germ cells was done by 5-methyl cytosine immunostaining.

Results

Streptozotocin administration successfully resulted in hyperglycemic response which significantly affected serum testosterone level, sperm DNA integrity and DNA ploidy at the end of 36 days. However, no changes were observed in either epididymal sperm concentration or germ cell methylation status. In contrast, at the end of 76 days, although serum testosterone level, sperm DNA integrity and DNA ploidy status were unperturbed significantly in hyperglycemic group, the epididymal sperm concentration and methylation status of preleptotene/zygotene cells were significantly altered. Importantly, an attempt to find out the association between the blood glucose levels and the abnormalities in hyperglycemic group failed to demonstrate any correlation.

Conclusions

The germ cell abnormalities observed in hyperglycemic group could be interpreted as a primary effect of streptozotocin and not due to hyperglycemia. Our results call for further evaluation of streptozotocin before its application to study the hyperglycemic responses on male germ cells.

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Acknowledgment

Authors would like to acknowledge technical support from Ms. Jayalaxmi Pai and Ms. Kirthi Patil (Clinical Embryology, Kasturba Medical College, Manipal).

This work was partially supported by the intramural funding from Kasturba Medical College, Manipal to RB (Grant # PGR-012).

Author information

Authors and Affiliations

  1. Clinical Embryology, Obstetrics & Gynaecology, Kasturba Medical College, Manipal University, Manipal, 576 104, India

    Rohini Bose, Satish K. Adiga, Fiona D’Souza, Sujith R. Salian, Shubhashree Uppangala, Guruprasad Kalthur & Pratap Kumar

  2. National Centre for Biological Sciences, Bangalore, India

    Navya Jain & Hanumantappa Krishnamurthy

  3. Department of Oral Pathology, Manipal College of Dental Sciences, Manipal University, Manipal, 576 104, India

    Raghu A. Radhakrishnan

  4. Department of Biochemistry, Kasturba Medical College, Manipal University, Manipal, 576 104, India

    Nalini Bhat

Authors
  1. Rohini Bose
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  2. Satish K. Adiga
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  3. Fiona D’Souza
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  4. Sujith R. Salian
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  5. Shubhashree Uppangala
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  6. Guruprasad Kalthur
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  7. Navya Jain
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  8. Raghu A. Radhakrishnan
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  9. Nalini Bhat
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  10. Hanumantappa Krishnamurthy
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  11. Pratap Kumar
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Corresponding author

Correspondence to Satish K. Adiga.

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Capsule

The germ cell abnormalities observed in streptozotocin induced hyperglycemic mice suggest possible effect of the drug and not due to hyperglycemic response.

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Bose, R., Adiga, S.K., D’Souza, F. et al. Germ cell abnormalities in streptozotocin induced diabetic mice do not correlate with blood glucose level. J Assist Reprod Genet 29, 1405–1413 (2012). https://doi.org/10.1007/s10815-012-9873-0

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  • DOI: https://doi.org/10.1007/s10815-012-9873-0

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