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The effect of long zona dissection using ICSI pipettes for mechanical assisted hatching in vitrified-thawed blastocyst transfers

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Abstract

Objective

To investigate the effect of long zona dissection (LZD) compared with partial zona dissection (PZD) using ICSI pipettes for mechanical assisted hatching (AH) in vitrified-thawed blastocyst transfers.

Design

Prospective study.

Setting

University IVF clinic.

Patient(s)

A total of 120 women ≦ 38 years old undergone vitrified-thawed blastocyst transfers with LZD or PZD.

Intervention(s)

The culture of all pronucleate embryos to the blastocyst stage and the selection of blastocysts ≧ grade 3BB (Gardner and Schoolcraft score), followed by vitrified-thawed blastocyst transfers with LZD (n = 60) or with PZD (n = 60)

Main outcome measure(s)

Complete hatching rates, implantation rates, pregnancy rates.

Result(s)

At 5 h after thawing, complete hatching rates of blastocysts were significantly higher in LZD group compared with PZD group, 52.4 % vs. 31.8 % (P = 0.001). Implantation and clinical pregnancy rates were significantly higher in LZD group compared with PZD group, 40.9 % vs. 25.7 % and 63.0 % vs. 40.0 %, respectively (P = 0.010, P = 0.011).

Conclusion(s)

LZD using ICSI pipettes for mechanical AH improves significantly complete hatching, implantation and pregnancy rates in vitrified-thawed blastocyst transfers.

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Acknowledgments

This study was supported by a Biomedical Research Institute Grant (2012-11), Pusan National University Hospital

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Pusan National University, School of Medicine, Gu-Duk-Ro 305, Su-Gu, Busan, Republic of Korea

    Sheng-Ta Sun, Jong-Ryeol Choi, Jung-Bin Son, Jong-Kil Joo & Kyu-Sup Lee

  2. Infertility Clinic, Pusan National University Hospital, Busan, South Korea

    Gyoung-Rae Ko

Authors
  1. Sheng-Ta Sun
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  2. Jong-Ryeol Choi
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  3. Jung-Bin Son
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  4. Jong-Kil Joo
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  5. Gyoung-Rae Ko
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  6. Kyu-Sup Lee
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Corresponding author

Correspondence to Kyu-Sup Lee.

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Capsule

Long zona dissection using ICSI pipettes for mechanical AH improves significantly hatching, implantation and pregnancy rates compared with partial zona dissection technique in vitrified-thawed blastocyst transfers.

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Sun, ST., Choi, JR., Son, JB. et al. The effect of long zona dissection using ICSI pipettes for mechanical assisted hatching in vitrified-thawed blastocyst transfers. J Assist Reprod Genet 29, 1431–1434 (2012). https://doi.org/10.1007/s10815-012-9872-1

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  • DOI: https://doi.org/10.1007/s10815-012-9872-1

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