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A review of, and commentary on, the ongoing second clinical introduction of preimplantation genetic screening (PGS) to routine IVF practice

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Current re-introduction of “improved” preimplantation genetic screening (PGS#2) raises the question whether PGS#2 is ready for routine clinical application.


We assessed available evidence via review of published data for years 2005–2012, and review of currently ongoing registered clinical trials, based on searches under appropriate key words in PubMed, MEDLINE, Cochrane Database System Review and Google Scholar and In absence of prospective clinical trials, and due to limited available data, individual publications/ongoing studies are assessed.


PGS#2 offers significant improvements in accuracy of aneuploidy diagnosis over PGS#1. By moving embryo biopsy from day-3 after fertilization (6–8 cell stage) to trophectoderm biopsy at blastocyst stage (day 5–6), PGS#2, however, adds additional co-variables to the analysis of efficacy of the procedure, which have special relevance for women with diminished ovarian reserve (DOR), who usually produce small egg and embryo numbers. Limited published data, claiming efficacy of PGS#2, as well as ongoing clinical trials, do not consider these additional co-variables, do not analyze outcomes by intent to treat and, therefore, have to be considered biased in patient selection.


Here reached conclusions are based on absence of adequate data rather than affirmative outcome assessments. They, therefore, are subject to change at any future date with generation of significant new data. Premature introduction of PGS#1 caused significant damage to patients. As currently no reliable PGS#2 data are available to suggest improvements in IVF outcomes, to avoid a repeat of the PGS#1 experience, PGS#2 should be considered experimental until data show otherwise.

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  1. Gleicher N, Weghofer A, Barad D. Preimplantation genetic screening: “established” and ready fro prime time? Fertil Steril. 2008;89:780–8.

    Article  PubMed  Google Scholar 

  2. Mastenbroek S, Twisk M, van Echten-Arends J, Sikkema-Raddatz B, Korevaar JC, Verhoeve HR, et al. In vitro fertilization with preimplantation genetic screening. N Engl J Med. 2007;357:9–17.

    Article  PubMed  CAS  Google Scholar 

  3. Hardarson T, Hanson C, Lundin K, Hillensjö T, Nilsson L, Stevic J, et al. Preimplantation genetic screening in women of advanced maternal age caused a decrease in clinical pregnancy rate: a randomized controlled trial. Hum Reprod. 2008;23:2806–12.

    Article  PubMed  CAS  Google Scholar 

  4. Mayer LR, Klipstein S, Hazlett WD, Nasta T, Mangan P, Karande V. A prospective randomized controlled trial of preimplantation genetic screening in the “good prognosis” patient. Fertil Steril. 2009;91:1731–8.

    Article  Google Scholar 

  5. Debrock S, Melotte C, Spiessens C, Peeraer K, Vanneste E, Meeuwis L, et al. Preimplantation genetic screening for aneuploidy of embryos after in vitro fertilization in women aged at least 35 years: a prospective randomized trial. Fertil Steril. 2010;93:364–73.

    Article  PubMed  Google Scholar 

  6. Mastenbroek S, Twisk M, van der Veen F, Repping S. Preimplantation genetic screening: a systematic review and meta-analysis of RCTs. Huma Reprod Update. 2011;17:454–4566.

    Article  CAS  Google Scholar 

  7. Anderson RA, Pickering S. The current status of preimplantation genetic screening: British Fertility Society Policy and Practice Guidelines. Hum Fertil (Camb). 2008;11:71–5.

    Article  Google Scholar 

  8. Practice Committee of Society for Assisted Reproductive Technology, Practice Committee of American Society for Reproductive Medicine. Preimplantation genetic testing: a practice committee opinion. Fertil Steril. 2008;90(5 Suppl):S136–43.

    Google Scholar 

  9. Harper J, Coonen E, de Rycke M, Fiorentino F, Geraedts J, Goossens V, et al. What next for preimplantation genetic screening (PGS)? A position statement from the ESHRE PGD Consortium Steering Commmitttee. Hum Reprod. 2010;25:821–3.

    Article  PubMed  Google Scholar 

  10. Gleicher N, Kim A, Weghofer A, Barad DH. Lessons from elective in vitro fertilization (IVF) in, principally, non-infertile women. Reprod Biol Endocrinol. In press

  11. Fragouli E, Katz-Jaffe M, Alfarawati S, Stevens J, Colls P, Goodall NN et al. Comprehensive chromosome screening of polar bodies and blastocysts from couples experiencing repeated implantation failure. Fertil Steril. ;94:875–87.

  12. Schoolcraft WB, Fragouli E, Stevens J, Munné S, Katz-Jaffe MG, Wells D. Clinical application of comprehensive chromosomal screening at the blastocyst stage. Fertil Steril. 2010;94:1700–6.

    Article  PubMed  Google Scholar 

  13. Rius M, Daina G, Obradors A, Ramos L, Velilla E, Fernández S, et al. Comprehensive embryo analysis of advanced maternal age-related aneuploidies and mosaicism by short genomic hybridization. Fertil Steril. 2011;95:413–6.

    Article  PubMed  Google Scholar 

  14. Brezina PR, Benner A, Rechitsky S, Kuliev A, Pomerantseva E, Pauling D, et al. Single-gene testing combined with single nucleotide polymorphism microarray preimplantation genetic diagnosis for enuploidy: a novel approach in optimizing pregnancy outcome. Fertil Steril. 2011;95:1786e5–8.

    Google Scholar 

  15. Scott Jr RT, Ferry K, Su J, Tao X, Scott K, Treff NR. Comprehensive chromosome screening is highly predictive of the reproductive potential of human embryos: a prospective, blinded, nonselection study. Fertil Steril. 2012;97:870–5.

    Article  PubMed  Google Scholar 

  16. Yang Z, Liu J, Collins GS, Salem SA, Liu X, Lyle SS, et al. Selection of sinle blastocysts for fresh transfer via standard morphology assessment alone and with array CGH for good prognosis IVF patients: results from a randomized pilot study. Mol Cytogenet. 2012;5:24.

    Article  PubMed  Google Scholar 

  17. Milán M, Cobo AC, Rodrigo L, Mateu E, Mercader A, Buendia P, et al. Redefining advanced maternal age as an indication for preimplantation geneic screening. Reprod Biomed Online. 2010;21:649–57.

    Article  PubMed  Google Scholar 

  18. Rabinowitz M, Ryan A, Gemelos G, Hill M, Baner J, Cinnioglu C, et al. Origins and rates of aneuploidy in human blastomeres. Fertil Steril. 2012;97:395–401.

    Article  PubMed  Google Scholar 

  19. Treff NR, Tao X, Ferry KM, Su J, Taylor D, Scott Jr RT. Development and validation of an accurate quantitative real-time polymerase chain reaction-based assay for human blastocyst comprehensive chromosomal aneuploidy screening. Fertil Steril. 2019;97:819–24.

    Article  Google Scholar 

  20. Verlinsky Y, Kuliev A. Preimplantation diagnosis for aneuploidies in assisted reproduction. Minerva Ginecol. 2004;56:197–203.

    PubMed  CAS  Google Scholar 

  21. Kuliev A, Cieslak J, Verlinsky Y. Frequency and distribution of chromosome abnormalities in human oocytes. Cytogenet Genome Res. 2005;111:193–8.

    Article  PubMed  CAS  Google Scholar 

  22. Verlinsky Y, Cieslak J, Freidine M, Ivakhnenko V, Wolf G, Kovalinskaya L, et al. Polar body diagnosis of common aneuploidies by FISH. J Assist Reprod Genet. 1996;13:57–162.

    Google Scholar 

  23. Verlinsky Y, Cieslak J, Ivakhnenko V, Evsikov S, Wolf G, White M, et al. Prepregnancy genetic testing for age-related aneuploidies by polar body analysis. Genet Test. 1997–1998;1:231–5.

    Google Scholar 

  24. Cohen J, Wells D, Munné S. Removal of 2 cells from cleavage stage embryos is likely to reduce the efficacy of chromosomal tests that are used to enhance implantation rates. Fertil Steril. 2007;87:496–503.

    Article  PubMed  Google Scholar 

  25. Baart EB, Martini E, van den Berg I, Macklon NS, Galijaard RJ, Fauser BC, et al. Preimplantation genetic screening reveals a high incidence of aneuploidy and mosaicism in embryos from young women undergoing IVF. Hum Reprod. 2006;21:223–33.

    Article  PubMed  CAS  Google Scholar 

  26. Munné S, Fragouli E, Colls P, Katz-Jaffe M, Schoolcraft W, Wells D. Improved detection of aneuploid blastocysts using a new 12-chromosome FISH test. Reprod Biomed Online. 2010;20:92–7.

    Article  PubMed  Google Scholar 

  27. McArthur SJ, Leigh D, Marshall JT, de Boer KA, Jansen RP. Oregnancies and live births after trophectoderm biopsy and preimplantation genetic testing of human blastocysts.

  28. Gutiérrez-Mateo C, Wells D, Benet J, Sánchez-García JF, Bermúdez MG, Belil I, et al. Reliability of comparative genomic hybridization to detect chromosome abnormalities in first polar bodies and metaphase II oocytes. Hum Reprod. 2004;19:2118–25.

    Article  PubMed  Google Scholar 

  29. Geraedts J, Collins J, Gianaroli L, Goossens V, Handyside A, Harper J, et al. What next for preimplantation genetic screening? A polar body approach? Hum Reprod. 2010;25:575–7.

    Article  PubMed  Google Scholar 

  30. Harper JC, Harton G. The use of arrays in preimplantation genetic diagnosis and screening. Fertil Steril. 2010;94:1173–7.

    Article  PubMed  CAS  Google Scholar 

  31. Schoolcraft WB, Treff NR, Stevens JM, Ferry K, Katz-Jaffe M, Scott Jr RT. Live birth outcome with trophectoderm biopsy, blastocyst vitrification, and single-nucleotide polymorphism microarray-based comprehensive chromosome screening in infertile patients. Fertil Steril. 2011;96:638–40.

    Article  PubMed  Google Scholar 

  32. Schoolcraft WB, Katz-jaffe MG, Stevens J, Rawlins M, Munné S. Preimplantation aneuploidy testing for infertile patients of advanced maternal age; a randomized prospective trial. Fertil Steril. 2009;92:157–62.

    Article  PubMed  Google Scholar 

  33. Twisk M, Mastenbroek S, van Wely M, Heineman MJ, Van der Veen F, Repping S. Preimplantation genetic screening for abnormal number of chromosomes (aneuploidies) in in vitro fertilization or intracytoplasmic sperm injection. Cochrane Database Syst Rev. 2006;(1):CD005291

  34. Musters AM, Repping S, Korevaar JC, Mastenbroek S, Limpens J, van der Veen F, et al. Pregnancy outcome after preimplantation genetic screening or natural conception in couples with unexplained recurrent miscarriages: a systematic review of the best available evidence. Fertil Steril. 2011;95:2153–7.

    Article  PubMed  Google Scholar 

  35. Ly KD, Agarwal A, Nagy ZP. Preimplantation genetic screening: does it help or hinder IVF treatment and what is the role of the embryo? J Assist Reprod Genet. 2011;28:833–49.

    Article  PubMed  Google Scholar 

  36. Harper JC, Sengupta SB. Preimplantation genetic diagnosis: state of the art 2011. Hum Genet. 2012;131:175–86.

    Article  PubMed  Google Scholar 

  37. Blake DA, Farquhar CM, Johnson N, Proctor M. Cleavage stage versus blastocyst stage embryo transfer in assisted conception. Cochrane Database Syst Rev. 2007;CD002118

  38. Barad DH, Weghofer A, Gleicher N. Age-specific levels for basal follicle-stimulating hormone assessment of ovarian function. Obstet Gynecol. 2007;109:1404–10.

    Article  PubMed  CAS  Google Scholar 

  39. Barad DH, Weghofer A, Gleicher N. Utility of age-specific serum anti-Müllerian hormone concentrations. Reprod Biomed Online. 2011;22:284–91.

    Article  PubMed  CAS  Google Scholar 

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This study was supported by extramural funds from the Foundation for Reproductive Medicine and intramural funds from the Center for Human Reproduction (CHR) New York.

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The authors have no conflict to declare in regards to here published materials.

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Correspondence to Norbert Gleicher.

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Norbert Gleicher and David H. Barad contributed equally to the work.


Though the current re-introduction of preimplantation genetic screening (PGS) involves greatly improved aneuploidy testing of embryos, currently available data do not demonstrate outcome improvements for in vitro fertilization (IVF). Until such data become available, PGS, therefore, should be considered an experimental procedure.

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Gleicher, N., Barad, D.H. A review of, and commentary on, the ongoing second clinical introduction of preimplantation genetic screening (PGS) to routine IVF practice. J Assist Reprod Genet 29, 1159–1166 (2012).

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