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A comparative study on oxidative and antioxidative markers of serum and follicular fluid in GnRH agonist and antagonist cycles

Journal of Assisted Reproduction and Genetics volume 29, pages 1175–1183 (2012)Cite this article

Abstract

Objective

To determine whether concentrations of oxidative stress markers of follicular fluid and serum are different in GnRH agonist protocol from GnRH antagonist protocol.

Material and method

This was a cross-sectional study. Eighty-four women undergoing controlled ovarian stimulation with either GnRH agonist (n = 39) or GnRH antagonist protocols (n = 45) for IVF/ICSI treatment were assigned by a physician. Blood was obtained at the time of oocyte retrieval, and follicular fluid (FF) from the mature follicles of each ovary was centrifuged and frozen until analysis. Malondialdehyde (MDA), nitric oxide (NO), protein carbonyl (PC), hydroxyl proline (OH-P), sodium oxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GSH-Px), adenosine deaminase (ADA) and xanthine oxidase (XO) were assessed in the serum and follicular fluid of each participants.

Results

The mean serum concentrations of GSH-Px, GSH and MDA were lower in the GnRH antagonist group compared to GnRH agonist group, but mean serum SOD was higher in the GnRH antagonist group. The mean follicular SOD, ADA and NO were higher in GnRH antagonist group than GnRH agonist group. The IVF/ICSI outcomes were similar in both groups.

Conclusion(s)

GnRH antagonist protocol is associated with increased oxidative stress. The relation of GnRH analogues with oxidative stress and its implication in follicular growth needs to be addressed in further studies.

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Conflict of interest

The authors declare that there is no conflict of interest.

Author information

Affiliations

  1. Department of Obstetrics and Gynecology, School of Medicine, Inonu University, 44280, Malatya, Turkey

    Ebru Celik, Onder Celik, Ercan Yilmaz, Ilgin Turkcuoglu, Yavuz Simsek & Abdullah Karaer

  2. Department of Obstetric and Gynecology, School of Medicine, Firat University, Elazig, Turkey

    Banu Kumbak

  3. Department of Obstetric and Gynecology, Medi Guven Hospital, Manisa, Turkey

    Yagmur Minareci

  4. Department of Biochemistry, School of Medicine, Inonu University, 44280, Malatya, Turkey

    Elif Ozerol & Kevser Tanbek

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  1. Ebru Celik
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  2. Onder Celik
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Correspondence to Ebru Celik.

Additional information

Capsule GnRH antagonist may have negative effect on follicular environment. The oxidative stress markers may reflect the follicular environment and may be related to follicular development.

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Celik, E., Celik, O., Kumbak, B. et al. A comparative study on oxidative and antioxidative markers of serum and follicular fluid in GnRH agonist and antagonist cycles. J Assist Reprod Genet 29, 1175–1183 (2012). https://doi.org/10.1007/s10815-012-9843-6

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  • DOI: https://doi.org/10.1007/s10815-012-9843-6

Keywords

  • Oxidative stress
  • GnRH antagonist
  • GnRH agonist
  • IVF/ICSI