Abstract
Objective
To study the outcome of blastocysts showing expansion on day 5 and transferred on day 5 or 6, in comparison with those unexpanded and transferred on day 6.
Study Design
Prospective cohort of 221 women prepared for BET classified into three groups according to timing of blastocyst expansion and day of embryo transfer. Group I; with expanded blastocysts on day 5 having day 5 transfer, group II; with expanded blastocysts on day 5 having day 6 transfer and group III ; with delayed expansion undergoing day 6 BET.
Results
Implantation rates, pregnancy rates, ongoing pregnancy rates, and live birth rates in the first 2 groups were almost double the rates in the third group. The figures for implantation rates were 40 % in the first two groups vs. 19 % in the third group (P < 0.05). Pregnancy rates were 60.9 % and 64 % vs. 31.8 % (P < 0.05) and ongoing pregnancy/ live-birth rates were 52.3 % & 56 % vs. 27.3 %.
Conclusion
The current study reports better implantation and pregnancy rates with earlier expanding blastocysts regardless of the time of transfer.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Papanikolaou EG, Kolibianakis EM, Tournaye H, et al. Live birth rates after transfer of equal number of blastocysts or cleavage-stage embryos in IVF. A systematic review and metaanalysis. Hum Reprod. 2008;23(1):91–9.
Utsunomiya T, Ito H, Nagaki M, Sato J. A prospective, randomized study: day 3 versus hatching blastocyst stage. Hum Reprod. 2004;19:1598–603.
Bungum M, Bungum L, Humaidan P, Yding Andersen C. Day 3 versus day 5 embryo transfer: a prospective randomized study. Reprod Biomed Online. 2003;7:98–104.
Papanikolaou EG, Evangelos G, D’haeseleer E, et al. Live birth rate is significantly higher after blastocyst transfer than after cleavage-stage embryo transfer when at least four embryos are available on day 3 of embryo culture. A randomized prospective study. Hum Reprod. 2005;20:3198–203.
Sills ES, Palermo GD. Human blastocyst culture in IVF: current laboratory applications in reproductive medicine practice. Rom J Morphol Embryol. 2010;51(3):441–5.
Huisman GJ, Fauser BC, Eijkemans MJ, Pieters MH. Implantation rates after in vitro fertilization and transfer of a maximum of two embryos that have undergone three to five days of culture. Fertil Steril. 2000;73:117–22.
Ziebe S, Petersen K, Lindenberg S, Andersen AG, Gabrielsen A, Andersen AN. Embryo morphology or cleavage stage: how to select the best embryos for transfer after in-vitro fertilization. Hum Reprod. 1997;12:15459.
Hsu MI, Mayer J, Aronshon M, et al. Embryo implantation in in vitro fertilization and intracytoplasmic sperm injection: impact of cleavage status, morphology grade, and number of embryos transferred. Fertil Steril. 1999;72:679–85.
Shapiro BS, Richter KS, Harris DC, Daneshmand ST. A comparison of day 5 and day 6 blastocyst transfers. Fertil Steril. 2001;75:1126–30.
Shoukir Y, Chardonnens D, Campana A, Bischof P, Sakkas D. The rate of development and time of transfer play different roles in influencing the viability of human blastocysts. Hum Reprod. 1998;13:676–81.
Shapiro BS, Daneshmand ST, Garner FC, Aguirre M, Ross R. Contrasting patterns in in vitro fertilization pregnancy rates among fresh autologous, fresh oocyte donor and cryopreserved cycles with the use of day 5 or day 6 blastocysts may reflect differences in embryo-endometrium synchrony. Fertil Steril. 2008;89(1):20–6.
Barrenetxea G, de Larruzea A, Ganzabal T, Jiménez R, Carbonero K, Mandiola M. Blastocyst culture after repeated failure of cleavage stage embryo transfers: a comparison of day 5 and day 6 transfers. Fertil Steril. 2005;83:49–53.
Van Voorhis B, Dorkas A. Delayed blastocyst transfer: is the window shutting. Fertil Steril. 2008;89(1):31–2.
McArthur S, Don Leigh D, Marshal J, App B, de Boer K, Jansen R. Pregnancies and live births after trophectoderm biopsy and preimplantation genetic testing of human blastocysts. Fertil Steril. 2005;84:1628–36.
McArthur SJ, Leigh D, Marshall JT, Gee AJ, De Boer KA, Jansen RP. Blastocyst trophectoderm biopsy and preimplantation genetic diagnosis for familial monogenic disorders and chromosomal translocations. Prenat Diagn. 2008;28(5):434–42.
Shapiro BS, Daneshmand ST, Garner FC, Aguirre M, Thomas S. Large blastocyst diameter, early blastulation, and low preovulatory serum progesterone are dominant predictors of clinical pregnancy in fresh autologous cycles. Fertil Steril. 2008;90(2):302–9.
Veeck LL, Bodine R, Clarke RN, Berrios R, Libraro J, Moschini RM, et al. High pregnancy rates can be achieved after freezing and thawing human blastocysts. Fertil Steril. 2004;82:1418–27.
Behr B, Gebhardt J, Lyon J, Milki AA. Factors relating to successful cryopreserved blastocyst transfer program. Fertil Steril. 2002;77:697–9.
Van Landuyt L, Stoop D, Verheyen G, Verpoest W, Camus M, Van de Velde H, Devroey P, Van den Abbeel E. Outcome of closed blastocyst vitrification in relation to blastocyst quality: evaluation of 759 warming cycles in a single-embryo transfer policy. Hum Reprod. 2011;26(3):527–34.
Author information
Authors and Affiliations
Corresponding author
Additional information
Capsule Expanded blastocysts on day 5 have good outcome whether transferred on day 5 or 6.
Rights and permissions
About this article
Cite this article
Elgindy, E., Elsedeek, M.SEA. Day 5 expanded blastocysts transferred on same day have comparable outcome to those left for more extended culture and transferred on day 6. J Assist Reprod Genet 29, 1111–1115 (2012). https://doi.org/10.1007/s10815-012-9837-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10815-012-9837-4