Journal of Assisted Reproduction and Genetics

, Volume 29, Issue 9, pp 861–867 | Cite as

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

  • Kishlay Kumar
  • Deepika Deka
  • Amar Singh
  • D. K. Mitra
  • B. R. Vanitha
  • Rima DadaEmail author



Standard semen parameters are poor predictors of fertility potential. To date, apart from, paternal karyotyping sperm factors are not evaluated in recurrent pregnancy loss (RPL), only recent studies have emphasized the role of sperm factors in early embryonic development as sperm transcribes genes critical for early embryonic development. Sperm DNA integrity is useful diagnostic and prognostic marker and has clinical implications in idiopathic recurrent pregnancy loss (iRPL) following spontaneous conception. The aim of this study was to assess DNA integrity in cases experiencing iRPL following spontaneous conception.


Semen samples from 45 patients and 20 controls were analyzed as per WHO 1999 guidelines and sperm chromatin structure assay (SCSA) was used to measure DNA fragmentation index (DFI).


By applying receiver operating curve (ROC) analysis, sperm DFI of approximately 26 % was found in male partner of couples experiencing iRPL.


Our data indicate that sperm from men with a history of iRPL have a higher percentage of DNA damage as compared to control group, and this can explain pregnancy loss in these patients. Men with higher DFI are infertile whereas men with lower DFI (26 %) are able to conceive but experience recurrent pregnancy loss. Thus it is important to evaluate sperm DFI in couples experiencing iRPL to understand exact aetiology of RPL and determine prognosis and management.


Idiopathic recurrent pregnancy loss SCSA DFI Sperm 



The grant and support from Department of Biotechnology (BT/PR13558/MED/30/282/2010) is highly acknowledged.

Declaration of conflicting interests

The author(s) declared no potential conflicts of interests with respect to the authorship and/or publication of this article.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Kishlay Kumar
    • 1
  • Deepika Deka
    • 2
  • Amar Singh
    • 3
  • D. K. Mitra
    • 3
  • B. R. Vanitha
    • 2
  • Rima Dada
    • 1
    Email author
  1. 1.Laboratory for Molecular Reproduction and Genetics, Department of AnatomyAll India Institute of Medical SciencesNew Delhi 29India
  2. 2.Department of Obstetrics and GynaecologyAll India Institute of Medical SciencesNew DelhiIndia
  3. 3.Department of Transplant ImmunologyAll India Institute of Medical SciencesNew DelhiIndia

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