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Value of sperm chromatin dispersion test in couples with unexplained recurrent abortion

  • Gamete Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Objective

To evaluate and compare standard sperm parameters and sperm chromatin integrity by sperm chromatin dispersion test (SCD) in ejaculates from men whose partners have a history of recurrent pregnancy loss and from control group of fertile men.

Methods

Thirty couples with unexplained recurrent abortion (case group) and 30 fertile couples (control group) referring to Shiraz infertility center were included. Sperm parameters were assessed in semen samples from two groups and then staining with SCD procedure. The results were analyzed by performing ANOVA and Tukey,s tests.

Results

In control group, nucleoids with big (65.93 ± 2.35), small (12.4 ± 0.60) and without halo (11.6 ± 0.50) showed significant difference with case group (41.40 ± 1.43), (21.16 ± 1.11) and (23.26 ± 1.10) respectively. In the RPL group spermatozoa with high percentage of abnormal parameters (morphology and motility) was observed (p ≤ 0.05).

Conclusion

This study strengthens the current literature associating sperm quality with recurrent pregnancy loss, and emphasizes the important of evaluating male factor by tests such as SCD in addition to conventional sperm parameters.

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Authors and Affiliations

  1. Department of Anatomical sciences, Ahvaz Jundishapor University of Medical Science, P.O. Box: 61357–15794, Ahvaz, Iran

    Forouzan Absalan

  2. Shiraz—Human Assisted Reproductive Center, Shiraz, Iran

    Forouzan Absalan, Alireza Ghannadi, Marjaneh Kazerooni, Rostami Parifar, Fatemeh Jamalzadeh & Sahar Amiri

  3. Islamic Azad University—larestan branch, Larestan, Iran

    Alireza Ghannadi

Authors
  1. Forouzan Absalan
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  2. Alireza Ghannadi
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  3. Marjaneh Kazerooni
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  4. Rostami Parifar
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  5. Fatemeh Jamalzadeh
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  6. Sahar Amiri
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Corresponding author

Correspondence to Forouzan Absalan.

Additional information

Capsule

Sperm quality is correlated with RPL and SCD test could be a good and cost-effective method for detection of extreme degree of DNA damage.

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Absalan, F., Ghannadi, A., Kazerooni, M. et al. Value of sperm chromatin dispersion test in couples with unexplained recurrent abortion. J Assist Reprod Genet 29, 11–14 (2012). https://doi.org/10.1007/s10815-011-9647-0

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  • DOI: https://doi.org/10.1007/s10815-011-9647-0

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