Journal of Assisted Reproduction and Genetics

, Volume 28, Issue 2, pp 157–166 | Cite as

Evaluation of maturation competence of metaphase II oocytes in mice based on the distance between pericentriolar materials of meiotic spindle

Distance of PCM during oocyte maturation
  • Chizuka SakaiEmail author
  • Yumi Hoshino
  • Yusuke Sato
  • Eimei Sato
Gamete Biology



To ascertain whether metaphase II (MII) spindle shape influences oocyte competence, we examined the meiotic spindle organization in in vivo ovulated (IVO) oocytes and in spontaneously matured or follicle stimulating hormone (FSH)-induced oocytes.


FSH-induced oocytes matured in Waymouth’s MB752/1 or human tubal fluid (HTF) media and oocytes matured spontaneously in the basal medium were obtained, and spindles were detected by immunofluorescence. To evaluate the fertilization-associated differences in spindle morphology, we performed in vitro fertilization and analysed integrin mRNA expression.


The distance between the pericentriolar materials (PCMs) in oocytes matured under all conditions was initially more, but it reduced gradually and increased again thereafter. Therefore, oocytes exhibiting a reduction in the distance between PCMs had the highest development rate to blastocyst in each condition.


These results indicate that the ‘maturation competence’ of MII oocytes can be evaluated on the basis of the distance between PCMs.


In vitro maturation Maturation competence Meiotic spindle Mouse oocyte Pericentriolar materials 



This work was supported by the Japan Society for the Promotion of Science Grant to E. Sato (No. 21248032). This work was also supported in part by Grant-in-Aid for Young Scientists (B) to Y. Hoshino (No. 21780250) from the Ministry of Education, Science, and Culture, Japan.


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Chizuka Sakai
    • 1
    Email author
  • Yumi Hoshino
    • 1
  • Yusuke Sato
    • 1
  • Eimei Sato
    • 1
  1. 1.Laboratory of Animal Reproduction, Graduate School of Agricultural ScienceTohoku UniversitySendaiJapan

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