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Association of creatin kinase B and peroxiredoxin 2 expression with age and embryo quality in cumulus cells

Journal of Assisted Reproduction and Genetics volume 27, pages 629–639 (2010)Cite this article

Abstract

Purpose

The purpose of this study was to identify age-related oocyte or embryo markers suitable for non-invasive analysis, as women over 38 years of age experience diminished pregnancy and ovulation rates.

Methods

We used real-time quantitative PCR to examine the gene expression profiles in cumulus cells acquired from older and younger age groups. We selected 11 genes involved in three functions that directly affect cellular aging: cell cycle control, apoptosis, and metabolism.

Results

CKB and PRDX2 were up-regulated in women older than 38 years, and the expression of these genes in cumulus cells was associated with embryo quality. In good-quality embryos, CKB expression was higher in the cumulus cells acquired from both older and younger age groups than in poor-quality embryos.

Conclusions

These potential relationships among cumulus cell gene expression, oocyte quality, and age may expand our understanding of oogenesis and embryo development. CKB and PRDX2 may serve as biomarkers or therapeutic targets for the developmental potential of oocytes.

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Acknowledgements

We thank Chung-I Chen, Chiu-Ping Chen, Hsiu-Hui Chen, and Ming-Chou Hung for their assistance in laboratory techniques. This study was supported by a research grant from National Science Council, Taiwan (NSC 93-2314-B -039-017 and NSC 97-2314-B-040-018) to Maw-Sheng Lee.

Financial support

This study was supported by a research grant from National Science Council, Taiwan (NSC 93-2314-B-039-017 and NSC 97-2314-B-040-018) to Maw-Sheng Lee.

Author information

Affiliations

  1. Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan

    Maw-Sheng Lee, Tsung-Hsien Lee & Lii-Shung Huang

  2. Department of Medicine, China Medical University, Taichung, Taiwan

    Maw-Sheng Lee

  3. Division of Infertility Clinic, Lee Womens’ Hospital, No. 263 Bei-Tun Road, Taichung, 406, Taiwan

    Maw-Sheng Lee, Tsung-Hsien Lee, Hui-Mei Wu, Chun-Chia Huang, Lii-Shung Huang & En-Hui Cheng

  4. Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung, Taiwan

    Maw-Sheng Lee, Chung-Hsien Liu & Tsung-Hsien Lee

  5. Department of Biotechnology, Central Taiwan University of Science and Technology, Taichung, Taiwan

    Chun-Chia Huang

  6. College of Life Science, National Chung Hsing University, Taichung, Taiwan

    Chuan-Mu Chen

  7. Department of Biochemistry, School of Medicine, Chung Shan Medical University, Taichung, Taiwan

    En-Hui Cheng

Authors
  1. Maw-Sheng Lee
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  2. Chung-Hsien Liu
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  8. En-Hui Cheng
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Correspondence to En-Hui Cheng.

Additional information

Capsule

In cumulus cell gene expression, Creatin kinase B and peroxiredoxin 2 may serve as biomarkers or therapeutic targets for the developmental potential of oocytes.

Where the work was done: Division of Infertility Clinic, Lee Women’s Hospital, Taichung, Taiwan.

Capsule In cumulus cell gene expression, Creatin kinase B and peroxiredoxin 2 may serve as biomarkers or therapeutic targets for the developmental potential of oocytes.

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Lee, MS., Liu, CH., Lee, TH. et al. Association of creatin kinase B and peroxiredoxin 2 expression with age and embryo quality in cumulus cells. J Assist Reprod Genet 27, 629–639 (2010). https://doi.org/10.1007/s10815-010-9459-7

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  • DOI: https://doi.org/10.1007/s10815-010-9459-7

Keywords

  • Aging
  • Creatin kinase B (CKB)
  • Cumulus cells
  • Embryo quality
  • Peroxiredoxin 2 (PRDX2)