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Kinetics of human male pronuclear development in a heterologous ICSI model

Journal of Assisted Reproduction and Genetics volume 27, pages 277–283 (2010)Cite this article

An Erratum to this article was published on 06 April 2010

Abstract

Purpose

To evaluate human sperm nuclear chromatin decondensation in a heterologous ICSI system using hamster ova injected with human sperm.

Materials and methods

Frozen hamster oocytes were injected with Triton X-100 treated sperm and fixed at different time points post ICSI. Oocytes injected with non-treated sperm served as controls. Male pronuclear decondensation was evaluated after staining with DAPI.

Results

Sperm cells with partially destroyed membranes and depletion of the acrosome decondense more rapidly and to a greater extent than membrane/acrosome intact cells. Marked variability in pronuclear size was observed for any time point post ICSI, which most probably reflects the heterogeneity in the mature human sperm population.

Conclusion

Remodeling of male gamete nuclei in this heterologous ICSI mimics events that occur during natural fertilization in humans and therefore this approach may be used for studies of human sperm chromosomes transformations.

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Author information

Affiliations

  1. The Jones Institute for Reproductive Medicine, Eastern Virginia Medical School, Norfolk, VA, 23507, USA

    Estella L. Jones, Olga Mudrak & Andrei O. Zalensky

  2. Institute of Cytology, Russian Academy of Sciences, St. Petersburg, 194064, Russia

    Olga Mudrak

Authors
  1. Estella L. Jones
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  2. Olga Mudrak
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  3. Andrei O. Zalensky
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Corresponding author

Correspondence to Andrei O. Zalensky.

Additional information

Capsule Human-hamster heterologous ICSI mimics sperm nuclei transformations that occur during natural fertilization in humans.

An erratum to this article can be found at http://dx.doi.org/10.1007/s10815-010-9410-y

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Jones, E.L., Mudrak, O. & Zalensky, A.O. Kinetics of human male pronuclear development in a heterologous ICSI model. J Assist Reprod Genet 27, 277–283 (2010). https://doi.org/10.1007/s10815-010-9402-y

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  • DOI: https://doi.org/10.1007/s10815-010-9402-y

Keywords

  • Acrosome
  • Chromatin
  • Decondensation
  • Heterologous ICSI
  • Pronucleus