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In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes

  • Fertility Preservation
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Abstract

Purpose

The purpose of this study was to evaluate the effects of cryotop vitrification of sheep cumulus-oocyte complexes (COCs) on oocyte maturation, apoptotic gene expression and incidence of chromosomal abnormalities.

Methods

Freshly isolated (control group) and vitrified-warmed COCs (cryotop group) were matured in vitro. The expression of pro- and anti-apoptotic genes was investigated by real-time PCR. The incidence of numerical chromosomal abnormalities was evaluated by cytogenetic analysis.

Results

The mean percentage of oocytes in the cryotop and control groups that reached metaphase II was 49.25 ± 3.01% and 51.94 ± 2.7% respectively. The expression rates of pro- and anti-apoptotic genes were similar in both groups, whereas the incidence of numerical chromosomal abnormalities was higher in the cryotop group compared to the control group (42.5% vs. 20%, p < 0.05).

Conclusion

Although cryotop vitrification of COCs did not affect the incidence of oocyte maturation or apoptotic gene expression, significant deficiencies in the maintenance of oocyte chromosomal organization were seen.

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Acknowledgements

This research was financially supported by Royan Institute. There is no conflict of interest in this article.

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Authors and Affiliations

  1. Department of Anatomy, Faculty of Medical Science, Tarbiat Modares University, P.O. Box: 14115-111, Tehran, Iran

    Bita Ebrahimi & Mojtaba Rezazadeh Valojerdi

  2. Department of Embryology, Royan Institute for Reproductive Biomedicine Research Center, ACECR, Tehran, Iran

    Mojtaba Rezazadeh Valojerdi & Poopak Eftekhari-Yazdi

  3. Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

    Hossein Baharvand

  4. Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran

    Hossein Baharvand

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  1. Bita Ebrahimi
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  2. Mojtaba Rezazadeh Valojerdi
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  3. Poopak Eftekhari-Yazdi
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  4. Hossein Baharvand
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Correspondence to Mojtaba Rezazadeh Valojerdi.

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Capsule

Apoptosis and chromosomal abnormality after oocyte vitrification.

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Ebrahimi, B., Valojerdi, M.R., Eftekhari-Yazdi, P. et al. In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes. J Assist Reprod Genet 27, 239–246 (2010). https://doi.org/10.1007/s10815-010-9401-z

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  • DOI: https://doi.org/10.1007/s10815-010-9401-z

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