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Peroxides in mineral oil used for in vitro fertilization: defining limits of standard quality control assays

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Abstract

Purpose

To determine the relative sensitivities of the 1 and 2-cell mouse embryo assays (MEA) and the human sperm motility assay (HSMA) for peroxides in mineral oil. The effect of peroxide on blastocyst cell number and apoptosis was also studied.

Methods

One and two-cell MEA and HSMA were performed using mineral oil containing cumene hydroperoxide (CH).

Results

The 1-cell MEA was twice as sensitive as the 2-cell MEA and 20-times more sensitive than the HSMA for CH in mineral oil. The sensitivity of the 1-cell MEA doubled when embryos were cultured individually versus group culture. CH decreased blastocyst cell number in a dose dependent manner.

Conclusions

Individually cultured 1-cell embryos had the highest sensitivity for peroxides in mineral oil. Current quality control assays, including group cultured murine embryos and human sperm motility, have limited sensitivity for peroxides in mineral oil and may not detect levels of peroxides that cause sub-lethal cellular damage.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Rochester, MN, USA

    Phoebe M. Hughes, Dean E. Morbeck, Susan B. A. Hudson & Charles C. Coddington

  2. Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street S.W., Rochester, MN, 55905, USA

    Dean E. Morbeck, Jolene R. Fredrickson & David L. Walker

  3. Department of Laboratory Medicine, Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Rochester, MN, USA

    David L. Walker

Authors
  1. Phoebe M. Hughes
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  2. Dean E. Morbeck
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  3. Susan B. A. Hudson
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  4. Jolene R. Fredrickson
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  5. David L. Walker
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  6. Charles C. Coddington
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Corresponding author

Correspondence to Dean E. Morbeck.

Additional information

Capsule

The 1-cell MEA is 20-times as sensitive as the HSMA for peroxides in oil. The sensitivity doubles when embryos are cultured individually.

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Hughes, P.M., Morbeck, D.E., Hudson, S.B.A. et al. Peroxides in mineral oil used for in vitro fertilization: defining limits of standard quality control assays. J Assist Reprod Genet 27, 87–92 (2010). https://doi.org/10.1007/s10815-009-9383-x

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  • DOI: https://doi.org/10.1007/s10815-009-9383-x

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