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Case report: successful delivery following the transfer of a human re-vitrified day-7 spontaneously hatched blastocyst developed from vitrified cleaved embryos

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Abstract

Purpose

To report a successful delivery after the transfer of a re-cryopreserved day-7 hatched blastocyst.

Methods and Results

A 30-year-old woman underwent a long-treatment protocol for ovarian stimulation. Fourteen mature oocytes were obtained, and twelve were fertilized. On day 3, two cleaved embryos were transferred, but no implantation occurred. The remaining embryos were vitrified. Subsequently, two vitrified day-3 embryos were transferred. The woman became pregnant and delivered a healthy baby. Subsequently, two vitrified day-3 embryos were transferred, but no pregnancy occurred. Subsequently, all the remaining vitrified day-3 embryos were cultured. On day 5, no blastocyst was obtained. The remaining embryos were continued to be cultured. On day 7, a hatched blastocyst was obtained and re-vitrified. Subsequently, the re-vitrified day-7 hatched blastocyst was transferred. The woman became pregnant and delivered a healthy female.

Conclusions

The day-7 hatched blastocyst developed from vitrified embryos can be re-vitrified and have pregnancy potential after re-warming.

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Authors and Affiliations

  1. Kinutani Women’s Clinic, 8-23-4F, Hondori, Naka-ku, Hiroshima, 730-0035, Japan

    Kenichiro Hiraoka, Kaori Hiraoka, Tomoyo Kusuda, Shinichiro Okano, Masayuki Kinutani & Kazuo Kinutani

  2. Graduate School of Applied Biosciences, Hiroshima Prefectural University, Hiroshima, 727-0023, Japan

    Toshitaka Horiuchi

Authors
  1. Kenichiro Hiraoka
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  2. Kaori Hiraoka
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  3. Toshitaka Horiuchi
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  4. Tomoyo Kusuda
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  5. Shinichiro Okano
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  6. Masayuki Kinutani
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  7. Kazuo Kinutani
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Corresponding author

Correspondence to Kenichiro Hiraoka.

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Capsule

Successful delivery was obtained following the transfer of a human re-vitrified day-7 spontaneously hatched blastocyst developed from vitrified cleaved embryos.

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Hiraoka, K., Hiraoka, K., Horiuchi, T. et al. Case report: successful delivery following the transfer of a human re-vitrified day-7 spontaneously hatched blastocyst developed from vitrified cleaved embryos. J Assist Reprod Genet 26, 405–409 (2009). https://doi.org/10.1007/s10815-009-9333-7

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  • DOI: https://doi.org/10.1007/s10815-009-9333-7

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