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Beneficial effect of desialylated erythropoietin administration on the frozen-thawed canine ovarian xenotransplantation

  • ANIMAL EXPERIMENTATION
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Abstract

Purpose

The main drawback of ovarian cryopreservation followed by transplantation is that a large proportion of follicles are lost after transplantation. Thus, effects of erythropoietin (EPO) and desialylated EPO administration on the frozen-thawed canine ovarian xenotransplantation were examined.

Methods

The protective and survival-promoting effects of EPO and desialylated EPO on the follicles of frozen-thawed canine ovaries after transplantation were examined using NOD-SCID mice. Frozen-thawed dog ovarian tissue with 400 U/kg of EPO or asialo EPO was placed into the ovarian bursa.

Results

At 4 weeks after the transplantation, the ovaries were removed and subjected to histological examination. The survival rate of early primary follicles was 15.2% in the EPO group and 157.6% in the asialo EPO group, in contrast to 10.1% in the untreated group.

Conclusions

These results demonstrate that administration of asialo EPO could be effectively used to enhance the survival of the follicles of transplanted cryopreserved ovaries.

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Acknowledgements

This study was supported by funds from the Ministry of Health, Labour and Welfare of Japan.

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Authors and Affiliations

  1. Research Unit for Functional Genomics, National Research Center of Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, 080-8555, Japan

    Hiroshi Suzuki, Takako Ishijima, Saori Maruyama, Yoshiko Yanagimoto Ueta & Yasuyuki Abe

  2. Chugai Pharmaceutical Co., Ltd., Nihonbashimuromachi, Tokyo, 103-8324, Japan

    Hideki Saitoh

Authors
  1. Hiroshi Suzuki
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  2. Takako Ishijima
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  3. Saori Maruyama
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  4. Yoshiko Yanagimoto Ueta
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Correspondence to Hiroshi Suzuki.

Additional information

Capsule Administration of asialo EPO enhanced the survival of the follicles of transplanted cryopreserved ovaries.

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Suzuki, H., Ishijima, T., Maruyama, S. et al. Beneficial effect of desialylated erythropoietin administration on the frozen-thawed canine ovarian xenotransplantation. J Assist Reprod Genet 25, 571–575 (2008). https://doi.org/10.1007/s10815-008-9271-9

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  • DOI: https://doi.org/10.1007/s10815-008-9271-9

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