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Protective potential of SCF for mice preimplantation embryos cultured in vitro in suboptimal conditions

  • Animal Experimentation
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To examine the effect of stem cell factor (SCF) to embryos exposed to detrimental factors.

Methods

Mice embryos cultured in control medium or Exp.1. with FasL or FasL+SCF Exp.2. with hydrogen peroxide (HP) or HP+SCF; Exp.3. frozen–thawed and cultured with or without SCF. Immunohistochemistry for Fas and c-kit receptors was performed in blastocysts. Blastocyst rates, total numbers of blastocyst cells (TB) and inner cell mass cell counts (ICM) were determined.

Results

Immunohistochemical studies revealed expression of both Fas and c-kit in blastocyst cells. Exp.1. Significantly more blastocysts were found in control when compared to FasL group and to FasL+SCF group. TB and ICM counts in control and FasL+SCF group were significantly higher comparing to FasL group. Exp.2. We found significant differences between three groups in all three evaluated parameters. The highest blastocyst rates, TB and ICM counts were found in control, lower in HP+SCF group and the worst in HP group. Exp.3. No significant differences in TB and ICM counts were found. More embryos formed blastocyst in control than in two cryopreserved groups. Blastocyst rates did not differ between two cryopreserved groups.

Conclusion

SCF may improve culture of embryos exposed to unfavorable milieu.

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Authors and Affiliations

  1. Department of Histology and Embryology, Pomeranian Medical University, 72 Powstancow Wlkp. St., 70-111, Szczecin, Poland

    Wojciech Glabowski & Barbara Wiszniewska

  2. Clinic for Reproduction and Gynecology, Pomeranian Medical University, Szczecin, Poland

    Wojciech Glabowski & Rafal Kurzawa

Authors
  1. Wojciech Glabowski
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  2. Barbara Wiszniewska
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  3. Rafal Kurzawa
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Corresponding author

Correspondence to Wojciech Glabowski.

Additional information

SCF—Stem cell factor has protective potential for mice preimplantation embryos exposed to Fas ligand or hydrogen peroxide but not for embryos that underwent freezing–thawing procedure.

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Glabowski, W., Wiszniewska, B. & Kurzawa, R. Protective potential of SCF for mice preimplantation embryos cultured in vitro in suboptimal conditions. J Assist Reprod Genet 25, 395–402 (2008). https://doi.org/10.1007/s10815-008-9248-8

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  • DOI: https://doi.org/10.1007/s10815-008-9248-8

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