Purpose: To investigate the effects of IVF aspiration on the temperature, pH, and dissolved oxygen of bovine follicular fluid.
Methods: The temperature was monitored at various positions in an aspiration kit. Dissolved oxygen and pH were measured before and after aspiration.
Results: The temperature of follicular fluid dropped by 7.7 ± 1.3°C upon aspiration. Dissolved oxygen levels rose by 5 ± 2 vol.%. The pH increased by 0.04 ± 0.01.
Conclusions: The temperature change was attributed mainly to evaporation of fluid in the collection tube. Changes in dissolved oxygen levels and pH were due to contact with air. Standard vacuum-based aspiration may induce changes in follicular fluid, which could be detrimental to oocyte health and affect attempts to correlate chemical characteristics with oocyte quality.
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ACKNOWLEDGMENTS
This work was done in partial fulfillment of a contract from the New Zealand Foundation for Research Science and Technology. We thank the staff at AFFCO Manawatu, New Zealand for their help in collecting bovine ovaries.
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Redding, G.P., Bronlund, J.E. & Hart, A.L. PALMERSTON NORTH, NEW ZEALAND The effects of IVF aspiration on the temperature, dissolved oxygen levels, and pH of follicular fluid. J Assist Reprod Genet 23, 37–40 (2006). https://doi.org/10.1007/s10815-005-9011-3
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DOI: https://doi.org/10.1007/s10815-005-9011-3