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Hydrocortisone UV-Vis Spectrophotometric Study: Stability, Determination of Its Acidity Constants and Quantification

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Journal of Applied Spectroscopy Aims and scope

Hydrocortisone is a glucocorticoid-type hormone produced naturally by the organism, such that it maintains the body functional during physical or mental stress episodes. The present work focuses on the UV-Vis spectrophotometric assessment of the chemical stability of hydrocortisone in an aqueous medium during the time of analysis, determination of its acidity constants through a robust methodology that involves the usage of SQUAD software and validation of an analytical method to determine hydrocortisone by means of UV-Vis spectrophotometry, in pharmaceuticals. The acidity constant was determined as pKa 11.45 ± 0.02 (298 K), and the molar absorptivity coefficients and the molar absorptivity coefficients of the species involved in this acidity equilibrium were also determined as a function of the wavelength. The analytical method developed attained detection and quantification limits of 386 ± 3 and 1280 ± 10 nM, respectively, whereas the validation allows reliable quantification of hydrocortisone in pharmaceuticals.

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Authors and Affiliations

  1. Universidad Autónoma Metropolitana-Azcapotzalco, Departamento de Materiales, Área Ingeniería de Materiales, México, México

    G. M. Ramos-Reyes, S. Corona-Avendaño, M. Romero-Romo & M. Palomar-Pardavé

  2. Universidad Autónoma Metropolitana-Iztapalapa, Departamento de Química, Área de Química Analítica, México, México

    M. T. Ramírez-Silva

Authors
  1. G. M. Ramos-Reyes
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  2. S. Corona-Avendaño
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Correspondence to S. Corona-Avendaño or M. T. Ramírez-Silva.

Additional information

Abstract of article is published in Zhurnal Prikladnoi Spektroskopii, Vol. 91, No. 1, p. 168, January–February, 2024.

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Ramos-Reyes, G.M., Corona-Avendaño, S., Ramírez-Silva, M.T. et al. Hydrocortisone UV-Vis Spectrophotometric Study: Stability, Determination of Its Acidity Constants and Quantification. J Appl Spectrosc 91, 218–227 (2024). https://doi.org/10.1007/s10812-024-01709-9

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  • DOI: https://doi.org/10.1007/s10812-024-01709-9

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