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Endogenous Photoacceptors Sensitizing Photobiological Reactions in Somatic Cells

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Journal of Applied Spectroscopy Aims and scope

The effects of radiation from laser and LED sources with wavelengths λ = 405 and 445 nm on the metabolic activity of cultured somatic cells were compared. A more pronounced inhibitory effect was observed upon exposure to light with λ = 405 nm. Fundamental differences in the action of monochromatic and quasi-monochromatic light were not noted. The photobiological effect was shown to be due to photochemical processes involving various reactive oxygen species, the contribution of which to cell inactivation depended on the time after the termination of irradiation. Porphyrin components were detected for the first time in fluorescence spectra of suspensions of living cells in addition to the flavin component. It was concluded based on a comparison of the absorption characteristics of flavin and porphyrin sensitizers, chemiluminescence analysis, and the biological effects of radiation with λ = 405 and 445 nm that endogenous porphyrins with the most intense absorption in this area made the determining contribution to singlet oxygen formation in cells when exposed to radiation with λ = 405 nm. The contribution of flavins was more pronounced under the action of radiation with λ = 445 nm, which corresponded to their absorption spectrum maximum and the absorption minimum of endogenous porphyrins.

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Authors and Affiliations

  1. B. I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, Minsk, Belarus

    V. Y. Plavskii, L. G. Plavskaya, O. N. Dudinova, A. I. Tretyakova, A. V. Mikulich, A. N. Sobchuk, R. K. Nahorny, T. S. Ananich, A. D. Svechko, S. V. Yakimchuk & I. A. Leusenko

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  1. V. Y. Plavskii
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Correspondence to V. Y. Plavskii.

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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 90, No. 2, pp. 239–252, March–April, 2023. https://doi.org/10.47612/0514-7506-2023-90-2-239-252.

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Plavskii, V.Y., Plavskaya, L.G., Dudinova, O.N. et al. Endogenous Photoacceptors Sensitizing Photobiological Reactions in Somatic Cells. J Appl Spectrosc 90, 334–345 (2023). https://doi.org/10.1007/s10812-023-01540-8

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