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Ultraviolet Spectrophotometric Method for the Determination of Amitriptyline Hydrochloride

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Journal of Applied Spectroscopy Aims and scope

Amitriptyline hydrochloride (AMH) is a first-generation tricyclic antidepressant drug. Higher doses of AMH may lead to undesirable clinical conditions such as cardiac arrhythmia, anxiety (sleep disturbances), tachycardia, convulsion, and hyperglycemia. Hence, the accurate analytical method for the quantification of AMH is crucial. The reported AMH quantification methods are less sensitive and require chromogenic reactions. A newly developed spectrophotometric method for the quantification of AMH in the aqueous medium (greener) without using chromogenic conditions is reported. The λmax of AMH is found to be 239 nm, and the method has been validated according to the International Conference on Harmonization Q2A guidelines. The linearity of the developed method is within the range 0.5–2.5 μg/ mL with the correlation coefficient (r2) of 0.9949, which indicates the higher sensitivity of the detection compared with the reported spectrophotometric methods. The percentage recovery (98–102%), precision, limit of detection (0.0266 μg/mL), and limit of quantification (0.0806 μg/mL) data also ensure the method efficiency. The forced degradation studies under the influence of various external factors also suggested the validity of the proposed method for routine analysis. The method was extended for the quantification of AMH present in marketed formulations.

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Authors and Affiliations

  1. Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Mysuru, India

    T. Durai Ananda Kumar & Md. Simon Haque

  2. JSS Academy of Higher Education & Research, Mysuru, Karnataka, India

    T. Durai Ananda Kumar & Md. Simon Haque

Authors
  1. T. Durai Ananda Kumar
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  2. Md. Simon Haque
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Correspondence to T. Durai Ananda Kumar.

Additional information

Published in Zhurnal Prikladnoi Spektroskopii, Vol. 89, No. 3, pp. 374–380, May–June, 2022.

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Kumar, T.D.A., Haque, M.S. Ultraviolet Spectrophotometric Method for the Determination of Amitriptyline Hydrochloride. J Appl Spectrosc 89, 476–481 (2022). https://doi.org/10.1007/s10812-022-01382-w

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  • DOI: https://doi.org/10.1007/s10812-022-01382-w

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