A simple, accurate, novel, safe, and precise method was developed for the estimation of erlotinib hydrochloride in tablet dosage form using a mixture of methanol and acetonitrile (50:50% V/V). The maximum and subsidiary peak absorption of erlotinib hydrochloride were noted at 247 and 333 nm, respectively. Erlotinib hydrochloride follows Beer's law in the concentration range of 5–30 μg/mL (r2 = 0.9992). In the proposed method, the subsidiary peak absorption wavelength of 333 nm was used to estimate the concentration of erlotinib hydrochloride in tablets. The linear regression equation was found to be y = 0.0461x + 0.0164. The developed method was validated according to the International Conference on Harmonization (ICH) guidelines, and the values of accuracy, precision, and other statistical variables were found to be in accordance with the prescribed values.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
http://www.accessdata.fda.gov/drugsatfda_docs/label/2008/021743s010lbl.pdf.
V. Ashok Chakravarthy and B. B. V. Sailaja, Europ. J. Pharm. Med. Res., 3, No. 12, 609–624 (2016).
K. Sereya, S. T. Latha, D. Kamalakannan, M. Jambulingam, M. Anilkumar, and S. Ananda Thangadurai, Int. J. Pharm. Health care Res., 1, No. 1, 23–27 (2013), www.ijphr.com.
M. Mathrusri, Annapurna, B. Venkatesh, and R. Krishna Chaitanya, Chem. Sci. Trans., 3, No. 2, 840–846 (2014), https://doi.org/10.7598/cst2014.828.
Camille Wermuth, David Aldous, Pierre Raboisson, and Didier Rognan, The Practice of Medicinal Chemistry, 4th ed. (2015), https://doi.org/10.1016/C2012-0-03066-9.
S. T. Latha, S. AnandaThangadurai, M. Jambulingam, K. Sereya, D. Kamalakannan, and M. Anilkumar. Arab. J. Chem., 10, No. 1, S1138–S1144 (2017), https://doi.org/10.1016/j.arabjc.2013.02.006.
Soheila Bolandnazar, Adeleh Divsalar, Hadi Valizadeh, Arash Khodaei, and Parvin Zakeri-Milani, Adv. Pharm. Bull., 3, No. 2, 289–293 (2013), https://doi.org/10.5681/apb.2013.047.
V. S. Saravanan and B. Mallikarjuna Rao, J. Drug Delivery Therap., 3, No. 1, 50–54 (2013).
Anna Svedberg, Henrik Green, Anders Vikström, Joakim Lundeberg, and Svante Vikingsson, J. Pharm. Biomed. Anal. 107, 186–195 (2015), https://doi.org/10.1016/j.jpba.2014.12.022.
Takahiro Suga, Miki Shimada, Masamitsu Maekawa, Hiroyuki Suzuki, Masaru Mori, Tatsuma Okazaki, Akira Inoue, Hiroaki Yamaguchi, and Nariyasu Mano, Chromatography, 38, 95–100 (2017), https://doi.org/10.15583/jpchrom.2017.007.
V. Rajesh, B. Anupama, V. Jagathi, and K. Varaprasad, Int. J. Biol. Med. Res., 2, No. 1, 433–435 (2011), www.biomedscidirect.com.
Int. Conf. Harmonization Guidelines, Validation of Analytical Procedures: Text and Methodology Q2(R1).
B. Babu, S. N. Meyyanathan, B. Gowramma, and S. T. Narenderan, Int. J. Chem. Tech. Res., 10, No. 15, 183–188 (2017), www.sphixsai.com.
Author information
Authors and Affiliations
Corresponding author
Additional information
Published in Zhurnal Prikladnoi Spektroskopii, Vol. 89, No. 3, pp. 369–373, May–June, 2022.
Rights and permissions
Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Jadav, R.A., Ambasana, M.A. & Bapodra, A.H. Development and Validation of the Ultraviolet Spectrophotometric Method for Determination of Erlotinib Hydrochloride. J Appl Spectrosc 89, 471–475 (2022). https://doi.org/10.1007/s10812-022-01381-x
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10812-022-01381-x