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Determination of α-Chymotrypsin-Catalyzed Reaction Rates at Different Temperatures and PH Values by Raman Spectroscopy

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Journal of Applied Spectroscopy Aims and scope

The rates of an enzymatic reaction are determined using spontaneous Raman spectroscopy. Time dependences of integrated Raman intensities in spectra of the reaction mixtures are analyzed. The rates of the α-chymotrypsincatalyzed reaction are determined at different temperatures and pH values.

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Authors and Affiliations

  1. Lomonosov Moscow State University, Moscow, 119991, Russia

    I. A. Balakhnina, N. N. Brandt, A. A. Mankova, A. Yu. Chikishev & I. G. Shpachenko

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  1. I. A. Balakhnina
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  2. N. N. Brandt
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  3. A. A. Mankova
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  4. A. Yu. Chikishev
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  5. I. G. Shpachenko
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Correspondence to I. G. Shpachenko.

Additional information

Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 88, No. 1, pp. 11–16, January–February, 2021.

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Balakhnina, I.A., Brandt, N.N., Mankova, A.A. et al. Determination of α-Chymotrypsin-Catalyzed Reaction Rates at Different Temperatures and PH Values by Raman Spectroscopy. J Appl Spectrosc 88, 6–11 (2021). https://doi.org/10.1007/s10812-021-01133-3

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  • DOI: https://doi.org/10.1007/s10812-021-01133-3

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