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Effect of the Hypoglycemic Agent Gliclazide on the Gastric Digestive Function: Binding Mechanism Between Gliclazide and Pepsin

Herein, the interaction between gliclazide (GCZ) and pepsin (PEP) was explored under simulated physiological conditions by multiple spectroscopy methods and molecular docking. The results showed that a new complex of 1:1 was formed between GCZ and PEP, thereby quenching the endogenous fluorescence of PEP. The addition of GCZ changed the conformation of PEP and increased the α-helix content in PEP from 20.16% to 21.13%. Using the binding constant Ka of the reaction between GCZ and PEP and the number of binding sites n, the binding rate formulas of GCZ and PEP were deduced. It was estimated that when the patient takes 40 mg of GCZ, the PEP in the gastric juice will be reduced by 96.69%. That meant taking GCZ will seriously affect the patient's digestive function. The results of molecular docking indicated that the GCZ binding site was located in the active center of PEP. The interaction of the two was driven by electrostatic attraction and hydrogen bonding forces.

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References

  1. Q. Tang, J. H. Su, H. Y. Cao, L. H. Wang, L. X. Gao, and X. F. Zheng, Spectrosc. Spectr. Anal., 37, No. 11, 3485–3492 (2017).

    Google Scholar 

  2. A. T. Buddanavar and S. T. Nandibewoor. J. Pharmac. Analysis, 7, No. 3, 148–155 (2017).

    Article  Google Scholar 

  3. T. Ahammad, M. Begum, A. F. M. T. Rahman, M. Hasan, S. R. Paul, S. Eamen, M. I. Hussain, M. H. Ali, M. A. Islam, M. M. Rahman, and M. Rashid, Pharmacol. Pharm., 6, No. 3, 125–131 (2015).

    Article  Google Scholar 

  4. Q. J. Zhang, B. S. Liu, G. X. Li, and R. Han, Luminescence, 31, No. 5, 1109–1114 (2016).

    Article  Google Scholar 

  5. Y. R. Wang, Q. Fang, C. H. Guo, and Y. Liu. Spectrosc. Spectr. Anal.,36, No. 10, 3414–3421 (2016).

    Google Scholar 

  6. G. J. Nan, P. Wang, J. Sun, J. H. Lv, M. W. Ding, L. Yang, Y. P. Li, and G. D. Yang, Luminescence, 31, No. 8, 1524–1531 (2016).

    Article  Google Scholar 

  7. Y. Q. Wang and H. M. Zhang, J. Agric. Food Chem., 61, No. 46, 11191–11200 (2013).

    Article  Google Scholar 

  8. R. Shahidha, S. Muthu, M. Raja, R. R. Muhamed, B. Narayana, P. S. Nayak, and B. K. Sarojini, Optik, 140, 1127–1142 (2017).

    Article  ADS  Google Scholar 

  9. J. K. Maurya, M. U. Mir, U. K. Singh, N. Maurya, N. Dohare, S. Patel, A. Ali, and R. Patel, Biopolymers, 103, No. 7, 406–415 (2015).

    Article  Google Scholar 

  10. N. Barbero, E. Barni, C. Barolo, P. Quagliotto, G. Viscardi, L. Napione, S. Pavan, and F. Bussolino, Dyes Pigments, 80, No. 3, 307–313 (2009).

    Article  Google Scholar 

  11. M. M. Yin, P. Dong, W. Q. Chen, S. P. Xu, L. Y. Yang, F. L. Jiang, and Y. Liu Langmuir,33, No. 21, 5108–5116 (2017).

    Article  Google Scholar 

  12. J. C. Li, N. Li, Q. H. Wu, Z. Wang, J. J. Ma, C. Wang, and L. J. Zhang. J. Mol. Struct., 833, Nos. 1–3, 184–188 (2007).

    Article  ADS  Google Scholar 

  13. L. L. He, X. Wang, B. Liu, J. Wang, Y. G. Sun, E. J. Gao, and S. K. Xu, J. Lumin., 131, No. 2, 285–290 (2011).

    Article  Google Scholar 

  14. Q. J. Zhou, J. F. Xiang, Y. L. Tang, J. P. Liao, C. Y. Yu, H. Zhang, L. Li, Y. Y. Yang, and G. Z. Xu, Colloids Surf. B, 61, No. 1, 75–80 (2008).

    Article  Google Scholar 

  15. W. J. Zhang, X. J. Xiong, F. Wang, L. Li, Y. Zhang, W. P. Xiao, and Y. Liu, Sci. Chin. Chem., 57, No. 12, 1690–1695 (2014).

    Article  Google Scholar 

  16. A. Jahanban-Esfahlan, V. Panahi-Azar, and S. Sajedi. Biopolymers, 103, No. 11, 638–645 (2015).

    Article  Google Scholar 

  17. Y. Q. Wang, X. Y. Wang, J. Wang, Y. M. Zhao, W. J. He, and Z. J. Guo. Inorg. Chem., 50, No. 24, 12661–12668 (2011).

    Article  Google Scholar 

  18. X. Y. Shi, H. Cao, F. L. Ren, and M. Xu. Chem. Biodivers, 4, No. 12, 2780–2790 (2007).

    Article  Google Scholar 

  19. X. J. Guo, X. D. Sun, and S. K. Xu, J. Mol. Struct., 931, Nos. 1–3, 55–59 (2009).

    Article  ADS  Google Scholar 

  20. X. R. Pan, P. F. Qin, R. T. Liu, and J. Wang, J. Agric. Food Chem., 59, No. 12, 6650–6656 (2011).

    Article  Google Scholar 

  21. X. Zhao, Y. L. Wang, J. L. Guo, H. R. Han, and M. S. Xie, Acta Sci. Circumstantiae, 25, No. 1, 52–57 (2005).

    Google Scholar 

  22. O. D. Putra, E. Yonemochi, and H. Uekusa. Cryst. Growth. Des., 16, No. 11, 6568–6673 (2016).

    Article  Google Scholar 

  23. J. P. Feng, X. Chen, R. L. Wang, and D. T. Yin, Chin. Med. Eng., 20, No. 3, 3–5 (2012).

    Google Scholar 

  24. X.Y. Gao, Y. C. Tang, W. Q. Rong, X. P. Zhang, W. J. Zhao, and Y. Q. Zi, Am. J. Anal. Chem., 2, No. 2, 250–257 (2011).

    Article  Google Scholar 

  25. F. L. Cui, Q. Z. Zhang, X. J. Yao, H. X. Luo, Y. Yang, L. X. Qin, G. R. Qu, and Y. Lu, Pestic. Biochem. Phys., 90, No. 2, 126–134 (2008).

    Article  Google Scholar 

  26. Y. X. Wu, Y. Qian, H. Cui, X. M. Lai, X. C. Xie, and X. R. Wang, Environ. Toxicol. Chem., 30, No. 12, 2697–2700 (2011).

    Article  Google Scholar 

  27. D. Yuan, Z. L. Shen, R. T. Liu, P. H. Wei, and C. Z. Gao, J. Chin. Chem. Soc., 61, No. 2, 255–262 (2014).

    Article  Google Scholar 

  28. N. Ibrahim, H. Ibrahim, S. Kim, J. P. Nallet, and F. Nepveu. Biomacromolecules, 11, No. 12, 3341–3351 (2010).

    Article  Google Scholar 

  29. M. M. Cui, B. S. Liu, T. T. Li, and S. T. Duan. Spectrosc. Lett., 49, No. 9, 573–581 (2016).

    Article  ADS  Google Scholar 

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Correspondence to B.-Sh. Liu.

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Abstract of article is published in Zhurnal Prikladnoi Spektroskopii, Vol. 86, No. 4, p. 671, July–August, 2019.

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Ma, LH., Liu, BS., Bian, G. et al. Effect of the Hypoglycemic Agent Gliclazide on the Gastric Digestive Function: Binding Mechanism Between Gliclazide and Pepsin. J Appl Spectrosc 86, 748–755 (2019). https://doi.org/10.1007/s10812-019-00889-z

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  • DOI: https://doi.org/10.1007/s10812-019-00889-z

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