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Investigation of the Regulatory Effect of 2-Hexadecenal on Neutrophils by the Chemiluminescence Method

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Journal of Applied Spectroscopy Aims and scope

The effect of 2-hexadecenal on the formation of reactive oxygen and chlorine species (ROCS) in neutrophils stimulated to phagocytosis has been determined by the chemiluminescence method. It has been established that at low concentrations this aldehyde demonstrates a priming effect on the cells, enhancing ROCS production, and at higher concentrations — significantly suppresses this process. Comparison of the results of chemiluminescence and fluorescence analysis of the cell characteristics suggests that 2-hexadecenal is a signaling molecule, which exhibits the properties of the neutrophil function regulator by modifying intracellular signaling processes associated with changes in ROCS production, cytoskeleton reorganization, increase in the level of unbound calcium ions in the cytoplasm, reduction of mitochondrial membrane potential. It also induces apoptosis.

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Authors and Affiliations

  1. Belarusian State University, 4 Nezavisimost′ Ave., 220030, Minsk, Belarus

    N. V. Amaegberi, G. N. Semenkova, A. G. Lisovskaya, S. S. Gusakova, V. А. Prokasheva & O. I. Shadyro

  2. Notre Dame Radiation Laboratory, University of Notre Dame, Notre Dame, Indiana, 46556, USA

    A. G. Lisovskaya

Authors
  1. N. V. Amaegberi
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  2. G. N. Semenkova
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  3. A. G. Lisovskaya
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  4. S. S. Gusakova
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  5. V. А. Prokasheva
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  6. O. I. Shadyro
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Correspondence to N. V. Amaegberi.

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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 86, No. 4, pp. 582–589, July–August 2019.

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Amaegberi, N.V., Semenkova, G.N., Lisovskaya, A.G. et al. Investigation of the Regulatory Effect of 2-Hexadecenal on Neutrophils by the Chemiluminescence Method. J Appl Spectrosc 86, 636–642 (2019). https://doi.org/10.1007/s10812-019-00871-9

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  • DOI: https://doi.org/10.1007/s10812-019-00871-9

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