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Development and Validation of an UV Spectrophotometric Method for Determination of Thiamphenicol in Dosage Form

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Journal of Applied Spectroscopy Aims and scope

A novel UV spectrophotometric method has been developed for the determination of thiamphenicol in a solid dosage form. The spectrophotometric detection was carried out at an absorption maximum wavelength of 224 nm using water as a solvent. The method was validated for specificity, linearity, precision, accuracy, robustness, and limit of detection (LOD) and limit of quantitation (LOQ). The detector response for thiamphenicol was linear in the selected concentration range of 5 to 25 μg/mL with a correlation coefficient of 0.9975. The precision (RSD) among six sample preparations was 0.65 and 1.50 % for analysts 1 and 2, respectively. The average recovery was 99.91 ± 0.65%. The LOD and LOQ were respectively 0.59 and 1.99 μg/mL. The results demonstrated that the excipients in thiamphenicol soft capsules did not interfere with the spectrophotometric determination of the drug. Using the Youden and Steiner approach, the method proved to be robust. The proposed method can be conveniently employed for routine analysis of thiamphenicol in bulk drug and soft capsules.

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Authors and Affiliations

  1. Universidade Federal do Ceará, Departamento de Farmácia, Faculdade de Farmácia, Odontologia e Enfermagem, Rua Capitão Francisco Pedro, 1210, Fortaleza, CE, CEP 60430-370, Brazil

    Y. A. Martins & C. L. C. G. De Oliveira

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  1. Y. A. Martins
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  2. C. L. C. G. De Oliveira
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Correspondence to C. L. C. G. De Oliveira.

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Published in Zhurnal Prikladnoi Spektroskopii, Vol. 86, No. 4, pp. 575–581, July–August, 2019.

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Martins, Y.A., De Oliveira, C.L.C.G. Development and Validation of an UV Spectrophotometric Method for Determination of Thiamphenicol in Dosage Form. J Appl Spectrosc 86, 629–635 (2019). https://doi.org/10.1007/s10812-019-00870-w

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  • DOI: https://doi.org/10.1007/s10812-019-00870-w

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