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Sers Imaging of Nasopharyngeal Carcinoma Markers Using an Antibody-Conjugated Gold Nanoparticles Probe

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Journal of Applied Spectroscopy Aims and scope

Surface-enhanced Raman scattering (SERS) nanotags as an ultrasensitive nanoprobe is becoming popular for the detection of biomarkers. Herein, antibody-conjugated gold nanoparticles (AuNPs) were used to target LMP2A in an LMP2A-infected CNE2 cell line. SERS maps showed that the LMP2A was distributed around the cell, which was consistent with the results of immunofl uorescence staining in the previous report. This location could be due to the specific binding of the bioconjugated nanotags to the receptors on the cell surface. However, the CNE2 cell line without LMP2A-infected showed no detectable signal at 1044 cm–1. The results demonstrated the potential feasibility of AuNPs nanotags as highly sensitive probes conjugated at the subcellular level for detection and localization of cancer markers in nasopharyngeal carcinoma (NPC).

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Authors and Affiliations

  1. School of Mechanical and Electrical Engineering, Shenzhen Institute of Information Technology, Shenzhen, 518172, China

    J.-H. Li, Y.-B. Du & Y.-Q. Zhou

  2. State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Department of Experimental Research, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China

    Y. Du, G.-K. Feng & M.-S. Zeng

Authors
  1. J.-H. Li
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  2. Y. Du
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  3. G.-K. Feng
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  4. Y.-B. Du
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  5. Y.-Q. Zhou
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  6. M.-S. Zeng
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Corresponding authors

Correspondence to J.-H. Li or M.-S. Zeng.

Additional information

Published in Zhurnal Prikladnoi Spektroskopii, Vol. 84, No. 5, pp. 731–736, September–October, 2017.

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Li, JH., Du, Y., Feng, GK. et al. Sers Imaging of Nasopharyngeal Carcinoma Markers Using an Antibody-Conjugated Gold Nanoparticles Probe. J Appl Spectrosc 84, 790–795 (2017). https://doi.org/10.1007/s10812-017-0546-8

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  • DOI: https://doi.org/10.1007/s10812-017-0546-8

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