EPR Spectroscopy in Environmental Lichen-Indication

The paramagnetic properties of lichens were investigated using EPR spectroscopy and Xanthoria parietina (L.) Th. Fr. as a case study. It was found that the concentration of paramagnetic centers in lichen thalli increased as the air-pollution level increased. Possible formation mechanisms of the paramagnetic centers in lichens were discussed. The efficiency of using EPR spectroscopy to study lichens as environmental quality indicators was demonstrated.

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  1. 1.

    M. I. Ibragimova, A. I. Chushnikov, V. N. Moiseev, V. Yu. Petukhov, E. P. Zheglov, and G. V. Cherepnev, Biofizika, 58, No. 2, 289–294 (2013).

    Google Scholar 

  2. 2.

    V. L. Sharygin, M. K. Pulatova, T. G. Shlyakova, I. N. Todorov, Yu. I. Mitrokhin, A. M. Vasserman, and N. D. Iordanov, Biofizika, 54, No. 2, 311–322 (2009).

    Google Scholar 

  3. 3.

    R. I. Khalilov, A. N. Nasibova, V. A. Serezhenkov, M. A. Ramazanov, M. K. Kerimov, A. A. Garibov, and A. F. Vanin, Biofizika, 56, No. 2, 364–371 (2011).

    Google Scholar 

  4. 4.

    S. E. Zhuravleva, P. V. Bondarenko, and E. M. Trukhan, Biofizika, 58, No. 2, 329–333 (2013).

    Google Scholar 

  5. 5.

    A. Jezierski, E. Bylinska, and M. R. D. Seaward, Atmos. Environ., 33, No. 28, 4629–4635 (1999).

    ADS  Article  Google Scholar 

  6. 6.

    Lichen-Indication Methods for Environmental Pollution, Methodical Guide [in Russian], Moscow (1997).

  7. 7.

    N. N. Krasnogorskaya, N. Yu. Tsvileneva, G. R. Minullina, and S. E. Zhuravleva, RU Pat. No. 2260934 C1, Sept. 27, 2005, ″Method for Evaluation of Atmosphere Contamination by Means of Lichens.

  8. 8.

    S. E. Zhuravleva and P. V. Bondarenko, RU Pat. No. 2549471, Feb. 27, 2015, ″Method for Determining Environment Quality by EPR-Spectroscopy of Lichens.

  9. 9.

    P. V. Bondarenko, ″Biophysical Characteristics of the Sensor Properties of Cryptogams Using Lichens as a Case Study, Candidate Dissertation in Biological Sciences, Moscow Phys.-Tech. Inst. (State Univ.), Dolgoprudnyi (2013).

  10. 10.

    S. E. Bogushevich and S. V. Matveichuk, Zh. Prikl. Spektrosk., 76, No. 3, 440–445 (2009) [S. E. Bogushevich and S. V. Matveichuk, J. Appl. Spectrosc., 76, 414–419 (2009)].

  11. 11.

    M. A. Sysoeva, V. R. Khabibrakhmanova, V. S. Minkin, V. S. Gamayurova, and V. E. Petrashen′, Khim. Rastit. Syr′ya, No. 4, 105–109 (2007).

  12. 12.

    RD 52.04.667-2005, Documents on Atmospheric Pollution Conditions in the Cities for Informing Public Authorities, Community, and Population. General Requirements to the Development, Construction, Statement, and Content [in Russian], Moscow (2006).

  13. 13.

    Review of Environmental Conditions and Pollution in the Russian Federation for 2015, Moscow (2016).

  14. 14.

    Report of the Ministry of Ecology and Natural Resources of Nizhegorod. Oblast. Condition of the Environment and Natural Resources of Nizhegorod. Oblast in 2011, N. Novgorod (2012).

  15. 15.

    Maximum allowable concentrations (MACs) of pollutants in the air of residential areas. Health standards, GN, Moscow (2003).

  16. 16.

    G. Sandmann and P. Boger, Plant Physiol., 66, No. 5, 797–800 (1980).

    Article  Google Scholar 

  17. 17.

    Yu. A. Vladimirov, Soros. Obraz. Zh., 6, No. 12, 14–19 (2000).

    Google Scholar 

  18. 18.

    J. W. Shive, Plant Physiol., 16, No. 3, 435–445 (1941).

    Article  Google Scholar 

  19. 19.

    K. A. Solhaug and Y. Gauslaa, Oecologia, 108, No. 3, 412–418 (1996).

    ADS  Article  Google Scholar 

  20. 20.

    V. A. Roginskii, Phenolic Antioxidants. Reactivity and Efficiency [in Russian], Nauka, Moscow (1988).

  21. 21.

    V. V. Ptushenko, V. A. Karavaev, M. K. Solntsev, and A. N. Tikhonov, Biofizika, 58, No. 2, 313–320 (2013).

    Google Scholar 

  22. 22.

    S. S. Malhotra and A. A. Khan, Biochem. Physiol. Pflanz., 178, Nos. 2–3, 121–130 (1983)

    Article  Google Scholar 

Download references

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Correspondence to S. E. Zhuravleva.

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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 84, No. 4, pp. 623–627, July–August, 2017.

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Bondarenko, P.V., Nguyet, L.T.B., Zhuravleva, S.E. et al. EPR Spectroscopy in Environmental Lichen-Indication. J Appl Spectrosc 84, 646–649 (2017). https://doi.org/10.1007/s10812-017-0523-2

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  • electron paramagnetic resonance
  • paramagnetic properties
  • lichens
  • lichen-indication
  • air pollution Index