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Spectrofluorimetric Method for Estimation of Curcumin in Rat Blood Plasma: Development and Validation

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Journal of Applied Spectroscopy Aims and scope

Curcumin is a medicinally important phytoconstituent of curcuminoids. The present study describes development of a simple method for estimation of curcumin in rat plasma. This method involves the use of spectrofluorimetry for evaluation of curcumin at 257 (Ex) and 504 nm (Em). Sample preparation involves only two steps: extraction of curcumin and drying the extract. Following this procedure, the samples are reconstituted with ethyl acetate, and relative fluorescence intensity is measured using a spectrofluorimeter. The method was validated as per CDER guidelines. The linearity of the method was found to be in the range of 100–500 ng/mL with accuracy and precision lying within 2% RSD. The LOD and LOQ were found to be 15.3 and 46.1 ng/mL, respectively. The method was applied for pharmacokinetic evaluation in rats, and AUC, Cmax, and Tmax were found to be 5580 ± 1006 h × ng/mL, 1526 ± 209 ng/mL, and 2.97 ± 0.28 h, respectively, with a plasma half-life of 1.14 ± 0.27 h.

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Authors and Affiliations

  1. M. S. University of Baroda, Vadodara, Gujarat, India

    J. Trivedi, H. Gandhi & S. P. Rathod

  2. Institute of Pharmacy, Nirma University, Ahmedabad, -382481, Gujarat, India

    B. Variya

Authors
  1. J. Trivedi
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  2. B. Variya
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  3. H. Gandhi
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  4. S. P. Rathod
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Correspondence to B. Variya.

Additional information

Published in Zhurnal Prikladnoi Spektroskopii, Vol. 82, No. 6, pp. 904–908, November–December, 2015.

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Trivedi, J., Variya, B., Gandhi, H. et al. Spectrofluorimetric Method for Estimation of Curcumin in Rat Blood Plasma: Development and Validation. J Appl Spectrosc 82, 980–984 (2016). https://doi.org/10.1007/s10812-016-0215-3

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  • DOI: https://doi.org/10.1007/s10812-016-0215-3

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