With a certain concentration of Michaelis buffer solution (pH 6.1), and in the presence of CTMAB, the fluorescence intensity of fluorescein, which is quenched by Pd 2+ , can be enhanced after adding a certain content of L-leucine. Thereby, we establish a fluorescence spectrometry method to detect the content of L-leucine, the added amount of which is proportional to the fluorescence enhancement. When the excitation slit width is 3 nm and the emission slit width is 5 nm, we obtain the following results: correlation coefficient R = 0.9981, linear range 0.125–1.375 mg/l, and detection limit 0.00028 mg/l.
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Abstract of article is published in in Zhurnal Prikladnoi Spektroskopii, Vol. 82, No. 1, p. 160, January–February, 2015.
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Cheng, D., Zhu, H. Determination of Levo-Rotatory Leucine by a Fluorescent Probe. J Appl Spectrosc 82, 157–163 (2015). https://doi.org/10.1007/s10812-015-0080-5
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DOI: https://doi.org/10.1007/s10812-015-0080-5